Autor(es):
Mostrar menos - |
Pinto, Ana P.
[1]
;
da Rocha, Alisson L.
[1]
;
Cabrera, Elisa M. B.
[2]
;
Marafon, Bruno B.
[3]
;
Kohama, Eike B.
[1]
;
Rovina, Rafael L.
[3]
;
Simabuco, Fernando M.
[4]
;
Bueno Junior, Carlos R.
[3]
;
de Moura, Leandro P.
[4]
;
Pauli, Jose R.
[4]
;
Cintra, Dennys E.
[4]
;
Ropelle, Eduardo R.
[4]
;
da Silva, Adelino S. R.
[3, 1]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[2] Univ Guadalajara, Hlth Sci Univ Ctr, Inst Translat Nutrigenet & Nutrigen, Dept Mol Biol & Genom, Guadalajara, Jalisco - Mexico
[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Campinas UNICAMP, Lab Mol Biol Exercise LaBMEx, Sch Appl Sci, Limeira, SP - Brazil
Número total de Afiliações: 4
|
Background: Based on the crosstalk of inflammation with apoptosis, autophagy, and endoplasmic reticulum (ER) stress, the main objective of this study was to explore the role of interleukin-6 (IL-6) on genes and proteins related to these phenomena in the livers of mice submitted to acute exhaustive exercise. Methods: Reverse transcription-quantitative polymerase chain reaction and immunoblotting technique were used to evaluate the livers of wild-type (WT) and IL-6 knockout (KO) mice at baseline (BL) and 3 h after the acute exhaustive physical exercise (EE). Results: Compared to the WT at baseline, the IL-6 KO had lower exhaustion velocity, mRNA levels of Mtor, Ulk1, Map1lc3b, and Mapk14, and protein contents of ATG5 and p-p70S6K/p70S6K. For the WT group, the EE decreased glycemia, mRNA levels of Casp3, Mtor, Ulk1, Foxo1a, Mapk14, and Ppargc1a, and protein contents of ATG5 and p-p70S6K/p70S6K, but increased mRNA levels of Sqstm1. For the IL-6 KO group, the EE decreased glycemia, mRNA levels of Casp3 and Foxo1a, and protein contents of pAkt/Akt and Mature/Pro IL-1beta, but increased mRNA levels of Sqstm1, and protein contents of p-AMPK/AMPK. Conclusion: The inhibition of the hepatic autophagy markers induced by the acute EE was attenuated in IL-6 KO mice, highlighting a new function of this cytokine. (AU) |