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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis

Texto completo
Autor(es):
Tomazi, Tiago [1, 2] ; Freu, Gustavo [1] ; Alves, Bruna Gomes [1] ; de Souza Filho, Antonio Francisco [3] ; Heinemann, Marcos Bryan [3] ; dos Santos, Marcos Veiga [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Anim Prod & Nutr, Milk Qual Res Lab Qualileite, Pirassununga - Brazil
[2] Cornell Univ, Sch Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY - USA
[3] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Lab Bacterial Zoonosis, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 14, n. 10 OCT 22 2019.
Citações Web of Science: 0
Resumo

A genotypic characterization of Streptococcus uberis isolated from clinical mastitis (CM) in dairy cows, and the association of Strep. uberis genotypes and antimicrobial susceptibility (AMS) was performed. A total of 89 isolates identified as Strep. uberis from 86 dairy cows with CM in 17 dairy herds of Southeastern Brazil were genotyped using random amplified polymorphic DNA (RAPD) analysis. After genotyping, two clusters (I and II) were created according to RAPD types. A commercial broth microdilution test was used to determine the susceptibility of Strep. uberis isolates to 8 antimicrobials (ampicillin, ceftiofur, cephalothin, erythromycin, penicillin, penicillin+novobiocin, pirlimycin and tetracycline). For each antimicrobial, we determined the minimal inhibitory concentrations that inhibit 50% (MIC50) and 90% (MIC90) of Strep. uberis strains. Differences in AMS among genotypic clusters were evaluated using mixed regression models. Overall, a great polymorphism (56 RAPD-types) was found among Strep. uberis isolates, although a higher genetic similarity (based on the PCR bands features) was observed within herds after genotypic clustering. No differences in AMS were observed among clusters. Strep. uberis isolated from bovine CM were resistant to most antimicrobials, with the exception of cephalothin and penicillin+novobiocin. (AU)

Processo FAPESP: 15/10332-6 - Estudo da diversidade molecular de linhagens de Staphylococcus aureus isolados de mastite bovina
Beneficiário:Marcos Bryan Heinemann
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/22286-3 - Perfil de agentes etiológicos causadores de mastite clínica e uso de antimicrobianos em rebanhos leiteiros
Beneficiário:Tiago Tomazi
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/17411-6 - Impacto econômico e perfil de agentes etiológicos da mastite bovina
Beneficiário:Marcos Veiga dos Santos
Linha de fomento: Auxílio à Pesquisa - Regular