Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

Autor(es)

Chehade, Chayrra ^[1] ; Amaral, Fernanda G. ^{[2, 3]} ; Branco, Giovana S. ^[1] ; Cassel, Monica ^[4] ; De Jesus, Lazaro W. O. ^[5] ; Costa, Fabiano G. ^[6] ; Bordin, Silvana A. ^[3] ; Moreira, Renata G. ^[7] ; Borella, I, Maria

Afiliação do(s) autor(es)

^[1] I, Univ Sao Paulo, Fish Endocrinol Lab, Dept Cell & Dev Biol, Biomed Sci Inst, Av Prof Lineu Prestes 1524, Lab 426, BR-05508000 Sao Paulo, SP - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Physiol & Biophys, Biomed Sci Inst, Sao Paulo - Brazil

^[4] Mato Grosso Fed Inst Educ Sci & Technol IFMT, Dept Educ, Anim Sci, Alta Floresta Campus, Alta Floresta, MG - Brazil

^[5] Fed Univ Alagoas UFAL, Lab Appl Anim Morphophysiol, Dept Histol & Embryol, Inst Biol Sci & Hlth, Maceio, Alagoas - Brazil

^[6] State Univ Northern Parana UENP, Dept Biol Sci, Jacarezinho, Parana - Brazil

^[7] Univ Sao Paulo, Lab Metab & Reprod Aquat Organisms, Dept Physiol, Inst Biosci, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Reproduction and Development; v. 87, n. 6 MAY 2020.
• Citações Web of Science: 0

Resumo

Gonadotropin-releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic-pituitary-gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full-length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lh beta messenger RNA (mRNA) expression but not fsh beta mRNA expression, and only GnRH2 was able to increase maturation-inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning. (AU)

• Processo FAPESP: 10/17586-0 - Caracterização molecular dos GnRHs de Astyanax altiparanae (Garutti e Britski, 2000), seu efeito in vivo, e sua expressão temporal ao longo do estímulo reprodutivo.
• Beneficiário: Chayrra Chehade Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/09567-8 - Clonagem molecular de GnRH de Astyanax altiparanae e seu efeito in vivo
• Beneficiário: Maria Ines Borella
• Modalidade de apoio: Auxílio à Pesquisa - Regular