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Protective Effects of Dietary Capsaicin on the Initiation Step of a Two-Stage Hepatocarcinogenesis Rat Model

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Sarmiento-Machado, Luis Manuel [1] ; Romualdo, Guilherme Ribeiro [2] ; Zapaterini, Joyce Regina [1] ; Tablas, Mariana Baptista [1] ; Henrique Fernandes, Ana Angelica [3] ; Moreno, Fernando Salvador [4] ; Barbisan, Luis Fernando [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Biosci Inst, Dept Morphol, UNESP, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Med Sch, Dept Pathol, UNESP, Botucatu, SP - Brazil
[3] Sao Paulo State Univ, Biosci Inst, Dept Chem & Biochem, UNESP, Botucatu, SP - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Citações Web of Science: 1

Capsaicin (CPS), an ingredient of Capsicum plants, has anti-inflammatory, antioxidant and antitumoral properties. The mechanisms of CPS on hepatocarcinogenesis preclinical bioassays are not described. Thus, the protective effects CPS were evaluated in the early stages of chemically-induced hepatocarcinogenesis. Male Wistar rats received diet containing 0.01% or 0.02% CPS for 3 weeks. Afterwards, animals received a dose of hepatocarcinogen diethylnitrosamine (DEN, 100 mg/kg body weight). From weeks 4-12, groups had their diet replaced by a 0.05% phenobarbital supplemented one to promote DEN-induced preneoplastic lesions. Animals were euthanized 24 h after DEN administration (n = 5/group) or at week 12 (n = 9/group). The estimated CPS intake in rats resembled human consumption. At the end of week 3, dietary 0.02% CPS attenuated DEN-induced oxidative damage and, consequently, hepatocyte necrosis by reducing serum alanine aminotransferase levels, liver CD68-positive macrophages, lipid peroxidation, while increasing antioxidant glutathione system. Additionally, 0.02% CPS upregulated vanilloid Trpv1 receptor and anti-inflammatory epoxygenase Cyp2j4 genes in the liver. Ultimately, previous 0.02% CPS intake decreased the number of GST-P-positive preneoplastic lesions at week 12. Thus, CPS attenuated preneoplastic lesion development, primarily by diminishing DEN-induced oxidative liver injury. Findings indicate that CPS is a promising chemopreventive agent when administered after and during the early stages of hepatocarcinogenesis. (AU)

Processo FAPESP: 18/19432-1 - Análise molecular e funcional da carcinogênese mamária relacionada à restrição proteica materna.
Beneficiário:Joyce Regina Zapaterini Rossi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/12015-0 - Cafeína, Trigonelina e Ácido Clorogênico: Modulação da expressão de miRNAs na Hepatocarcinogênese associada à Fibrose.
Beneficiário:Guilherme Ribeiro Romualdo
Linha de fomento: Bolsas no Brasil - Doutorado