| Texto completo | |
| Autor(es): |
Guedes, Wesley Nascimento
[1]
;
Lucena, Guilherme Nunes
[1, 2]
;
de Paula, Ariela Veloso
[3]
;
Costa Marques, Rodrigo Fernando
[2, 4]
;
Verbi Pereira, Fabiola Manhas
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista Unesp, Inst Quim, Inst Pesquisa Bioenergia IPBEN, BR-14800060 Araraquara, SP - Brazil
[2] Univ Estadual Paulista Unesp, Inst Quim, Grp Mat Magnet & Coloides, BR-14800060 Araraquara, SP - Brazil
[3] Univ Estadual Paulista Unesp, Fac Ciencias Farmaceut, Rodovia Araraquara Jau, Km 1, BR-14800903 Araraquara, SP - Brazil
[4] Univ Estadual Paulista Unesp, Ctr Monitoramento & Pesquisa Qualidade Combustive, Biocombustiveis Petr & Derivados Cempeqc, Inst Quim, BR-14800060 Araraquara, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 7, n. 26, p. 27-35, JAN-MAR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
This study describes the estimation of the enzymatic activity of endoglucanase based on the 3,5-dinitrosalicylic acid (DNS) method and carboxymethylcellulose (CMC) substrate using glucose as the analyte. As an alternative to measuring the colored product obtained by the reaction between glucose and DNS, a free software app for mobile devices was used for estimating the color intensity of solutions, replacing the need of a UV-Vis spectrophotometer (reference method). The app is able to convert images in color histograms to red (R), green (G) and blue (B) scales and to provide univariate and multivariate calculations of the image data. The chemometric technique partial least squares (PLS) was employed with the app and presented accurate results that were comparable to those of the reference method. A correlation coefficient of 0.983 was obtained for the linear range between 0.60 and 2.60 mg mL(-1) of glucose, and the standard error of cross-validation (SECV) was 0.219 mg mL(-1). The proposed method (imaging - PLS) was applied for samples of cellulase enzymes from Aspergillus niger and Trichoderma reesei, revealing an interesting alternative to estimate the enzymatic activity of endoglucanase. (AU) | |
| Processo FAPESP: | 18/18212-8 - Desenvolvimento de modelos quimiométricos para determinação de impurezas sólidas na matéria prima cana-de-açúcar utilizando o potencial da fluorescência de raios-X por energia dispersiva |
| Beneficiário: | Fabiola Manhas Verbi Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/01102-8 - EMU concedido no processo 2018/18212-8: espectrômetro de fluorescência de Raios-X |
| Beneficiário: | Fabiola Manhas Verbi Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |