Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents Streptococcus mutans Biofilm Accumulation

Autor(es)

Castillo Pedraza, Midian C. ^{[1, 2]} ; de Oliveira Fratucelli, Erick Dante ^[1] ; Ribeiro, Sabrina Marcela ^[1] ; Florez Salamanca, Elkin Jahir ^[1] ; Colin, Jaqueline Silva ^[1] ; Klein, I, Marlise

Afiliação do(s) autor(es)

^[1] I, Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Magdalena, Fac Hlth Sci, Santa Marta 470003 - Colombia

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 25, n. 9 MAY 2020.
• Citações Web of Science: 0

Resumo

Dental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 mu g/mL), myricetin (500 mu g/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. (AU)

• Processo FAPESP: 17/07408-6 - Avaliação de atividades antimicrobiana e anti-biofilme de extratos e frações de espécies vegetais sobre microrganismos patogênicos orais
• Beneficiário: Sabrina Marcela Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/05423-0 - Função de DNA extracelular e de ácidos lipoteicóicos na matriz extracelular de biofilmes cariogênicos
• Beneficiário: Marlise Inêz Klein Furlan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/26623-5 - Influência de Streptococcus mutans na construção da matriz durante o desenvolvimento de biofilmes microcosmos expostos a desafio nutricional
• Beneficiário: Elkin Jahir Florez Salamanca
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/00753-0 - Análises in vivo da virulência dos genes lytTS e operon dltABCD de Streptococcus mutans e modulação dos mesmo por terapia direcionada
• Beneficiário: Midian Clara Castillo Pedraza
• Modalidade de apoio: Bolsas no Brasil - Doutorado