Tailoring Protein PEGylation Reaction: An Undergraduate Laboratory Experiment

Autor(es)

Santos, Joao H. P. M. ^[1] ; Brumano, Larissa P. ^[1] ; Pessoa, Jr., Adalberto ^[1] ; Rangel-Yagui, Carlota O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem Pharmaceut Technol, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chemical Education; v. 97, n. 5, p. 1443-1447, MAY 12 2020.
• Citações Web of Science: 0

Resumo

Protein PEGylation refers to the covalent binding of polyethylene glycol (PEG) moieties to a protein and has been increasingly applied, mainly in the biopharmaceutical industry. This technique may increase bioavailability and stability by decreasing immunogenicity and proteolytic attack. Given the industrial interest and the relevance of this technique, an example of a common PEGylation reaction is herein proposed, using lysozyme as a model protein. This class is divided into three parts: the first one is composed by the PEGylation reaction per se, the second one aims at the analytical quantification of the PEGylation yield, and the third aims to understand the effects of PEGylation on protein activity. Students should gain skills in comprehending this type of chemical reaction, calculating reaction yields, and realizing the benefits of protein PEGylation. This experimental class comprises two lab classes with the expected duration of 2 h each and is intended for students in the third and fourth years of courses such as pharmaceutical sciences, biochemistry, chemistry, biotechnology, and correlated areas. (AU)

• Processo FAPESP: 17/21819-9 - Otimização e viabilidade econômica do processo de produção de L-asparaginase recombinante para aplicação farmacêutica
• Beneficiário: Larissa Pereira Brumano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/22065-5 - Pegilação N-terminal de proteínas e purificação por sistemas aquosos bifásicos
• Beneficiário: Carlota de Oliveira Rangel Yagui
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08617-7 - Produção de L-asparaginase extracelular: da bioprospecção à engenharia de um biofármaco antileucêmico
• Beneficiário: Adalberto Pessoa Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/25994-2 - Desenvolvimento de novas plataformas de PEGuilação de proteínas com potencial terapêutico com recurso à microfluídica
• Beneficiário: João Henrique Picado Madalena Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado