High p16(INK4a) immunoexpression is not HPV dependent in oral leukoplakia

Autor(es)

Tomo, Saygo ^{[1, 2]} ; Biss, Stephanye Pinto ^[1] ; Crivelini, Marcelo Macedo ^[2] ; Penha de Oliveira, Sandra Helena ^{[3, 1]} ; Biasoli, Eder Ricardo ^{[1, 2]} ; Tjioe, Kellen Cristine ^{[3, 1]} ; Bernabe, Daniel Galera ^{[1, 2]} ; Villa, Luisa Lina ^{[4, 5]} ; Miyahara, Glauco Issamu ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Oral Oncol Ctr, Sch Dent, Jose Bonifacio St 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Diag & Surg, Aracatuba - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Lab Pharmacol, Aracatuba, SP - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Radiol & Oncol, Sao Paulo, SP - Brazil

^[5] Inst Canc Estado Sao Paulo ICESP, Ctr Translat Oncol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 115, JUL 2020.
• Citações Web of Science: 0

Resumo

Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16(INK4a) immunoexpression in the northwest region of the Sao Paulo state, Brazil. Patients and methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 degrees C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16(INK4a) expression was performed. Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression. Conclusion: HPV was not identified in the population studied. The high p16(INK4a) immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions. (AU)

• Processo FAPESP: 16/12982-0 - Prevalência do hpv-16 e 18 no tecido e saliva de pacientes com cec de cabeça e pescoço e leucoplasia bucal e sua correlação com características clinicopatologicas e fatores prognósticos
• Beneficiário: Glauco Issamu Miyahara
• Linha de fomento: Auxílio à Pesquisa - Regular