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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Investigation of hemicellulosic hydrolysate inhibitor resistance and fermentation strategies to overcome inhibition in non-saccharomyces species

Texto completo
Autor(es):
Soares, L. B. [1, 2] ; Bonan, C. I. D. G. [1, 2] ; Biazi, L. E. [1, 2] ; Dionisio, S. R. [2] ; Bonatelli, M. L. [2] ; Andrade, A. L. D. [2] ; Renzano, E. C. [2] ; Costa, A. C. [1] ; Ienczak, J. L. [3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Chem Engn, UNICAMP, 500 Albert Einstein Av, BR-13083852 Campinas, SP - Brazil
[2] CTBE CNPEM, Brazilian Bioethanol Sci & Technol Lab, 10000 Giuseppe Maximo Scolfaro St, BR-13083852 Campinas, SP - Brazil
[3] Santa Catarina Fed Univ, Chem Engn & Food Engn Dept, CP 476, BR-88040900 Florianopolis, SC - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOMASS & BIOENERGY; v. 137, JUN 2020.
Citações Web of Science: 1
Resumo

Sugarcane bagasse, residue of first-generation ethanol fermentation, is a rich substrate for the second-generation (2G) ethanol. However, the processes used to release sugars from such material produce inhibitory compounds. The present study aims to elucidate the effects of inhibitory compounds present in hemicellulosic hydrolysates (acetic acid, furfural, 5-hydroxymethylfurfural and vanillin) on the yeast Spathaspora passalidarum and to compare its performance with that of the most commonly used yeast for 5-carbon consumption, Scheffersomyces stipitis. In addition, fermentation strategies such as using adapted strains and increasing the initial cell concentration were used to address the presence of inhibitors in hemicellulosic hydrolysates. The results showed that acetic acid and furfural are the strongest inhibitors of S. passalidarum. Interestingly, although ethanol production by S. passalidarum was strongly affected by all the considered inhibitors, the final concentrations of ethanol reached by this yeast were similar to those obtained by S. stipitis, even though S. stipitis was less inhibited. The adapted S. stipitis showed better performance than the wild type, resulting in a 25% increase in xylose consumption and a 5% increase in yield. Although increasing the initial concentration of cells improved the performance of the adapted S. stipitis, the performance of the S. passalidarum was better. The understanding of the behaviour of these yeasts in the presence of inhibitors allows to establish process strategies such as deciding when the use of high cell density is advantageous or selecting a methodology for the evolutionary adaptation of strains with the goal of higher performance in fermentation. (AU)

Processo FAPESP: 16/06142-0 - Estratégias para melhorar o desempenho do processo de produção de etanol de segunda geração
Beneficiário:Aline Carvalho da Costa
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular