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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes

Texto completo
Autor(es):
Camila Chioda de ALMEIDA ; Thaylane Paula FINANCI ; Marita Vedovelli CARDOZO ; Lucas Jose Luduverio PIZAURO ; Natalia PEREIRA ; Mylena Karoline VALMORBIDA ; Mariana Monezi BORZI ; Bruno WEISS ; Fernando Antônio de ÁVILA
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: FOOD SCIENCE AND TECHNOLOGY; n. ahead, p. -, 2020.
Resumo

Abstract The aim of this study was to investigate the presence of virulence and antimicrobial resistance genes and clonal profile of Enterobacteriaceae isolated from calves, cows, feeding buckets and the milk bucket. A a total of 31 Enterobacteriaceae isolates from calves (6), milk bucket (6), feeding buckets (6) and from cows’ rectum (13) were used. The presence of LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge as well as the antimicrobial resistance genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC and MCR-1 and SH were evaluated by PCR. The LT toxin gene were detected in five isolates (16.1%) and the mrkD gene was detected in three isolates (9.0%). The CTX-M-1 gene was detected in 13 isolates (41.9%), CTX-M-2 in five isolates (16.1%) and ACC-M in four isolates (12.9%). Most of the isolates obtained demonstrated, resistance to cephalothin (87.5%), ampicillin, (87.5%) and streptomycin (84.3%) with multidrug resistance observed in all isolates. Isolates from bucket and cow’s rectum, calves and milk bucket, calf and cow’s rectum shared the same pulsotypes. These findings may suggest that enterobacteria carring virulence and resistance genes may persist in the environment and became a reservoir of these genes. (AU)

Processo FAPESP: 19/02164-7 - Fatores de resistência a antimicrobianos: caracterização de AmpC, KPC e MCR-1 em enterobactérias isoladas na cadeia produtiva de frango de corte
Beneficiário:Fernando Antonio de Avila
Linha de fomento: Auxílio à Pesquisa - Regular