Improvement of Aglycone Content in Soy Isoflavones Extract by Free and Immobilized Beta-Glucosidase and their Effects in Lipid Accumulation

Autor(es)

Angelotti, Joelise A. F. ^[1] ; Dias, Fernanda F. G. ^[2] ; Sato, Helia H. ^[3] ; Fernandes, Pedro ^{[4, 5, 6]} ; Nakajima, Vania M. ^[7] ; Macedo, Juliana ^{[8, 9]}

Afiliação do(s) autor(es)

^[1] Univ Fed Alfenas, Inst Chem, R Gabriel Monteiro da Silva 700, Alfenas, MG - Brazil

^[2] Univ Calif Davis, Dept Food Sci & Technol, 2212 Robert Mondavi Inst South, Davis, CA 95616 - USA

^[3] Univ Estadual Campinas, UNICAMP, Fac Food Engn, Dept Food Sci, Monteiro Lobato 80, Cidade Univ, BR-13083862 Campinas, SP - Brazil

^[4] Univ Lisbon, Inst Bioengn & Biosci, Inst Super Tecn, Dept Bioengn, Av Rovisco Pais, P-1049001 Lisbon - Portugal

^[5] Univ Lusofona Humanidades & Tecnol, DREAMS, Av Campo Grande 376, P-1749024 Lisbon - Portugal

^[6] Univ Lusofona Humanidades & Tecnol, Fac Engn, Av Campo Grande 376, P-1749024 Lisbon - Portugal

^[7] Fluminense Fed Univ UFVF, Fac Nutr, Dept Nutr & Dietet, Rua Marios Santos Braga 30, BR-24020140 Niteroi, RJ - Brazil

^[8] Univ Estadual Campinas, UNICAMP, Fac Food Engn, Dept Food & Nutr, Rua Monteiro Lobato 80, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP, Brazil.Angelotti, Joelise A. F., Univ Fed Alfenas, Inst Chem, R Gabriel Monteiro da Silva 700, Alfenas, MG - Brazil

^[9] Univ Estadual Campinas, UNICAMP, Fac Food Engn, Dept Food & Nutr, Rua Monteiro Lobato 80, Cidade Univ Zeferino Vaz, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 192, n. 3 JUN 2020.
• Citações Web of Science: 1

Resumo

Soybean is one of the most important commodities in the world, being applied in feed crops and food, pharmaceutical industries in different ways. Soy is rich in isoflavones that in aglycone forms have exhibited significant anti-obesity and anti-lipogenic effects. Obesity is a global problem as several diseases have been related to this worldwide epidemic. The aim of this work was to verify the effect of free and immobilized beta-glucosidase, testing Lentikats, and sol-gel as carriers. Moreover, we wanted to examine if the different types of hydrolysis would generate extracts with distinct biological activity concerning lipid accumulation, PPAR-alpha regulation, and TNF-alpha, IL-6, and IL-10 concentrations using in vitro assays. Our results show that all formulations of beta-glucosidase could hydrolyze soy isoflavones. Thus, after 24 h of incubation, daidzein content increased 2.6-, 10.8-, and 12.2-fold; and genistein content increased 11.7, 11.4, and 11.4 times with the use of free enzyme, Lentikats (R), and sol-gel immobilized enzyme, respectively. Moreover, both methodologies for enzyme immobilization led to promising forms of biocatalysts for application in the production of soy extracts rich in isoflavones aglycones, which are expected to bring about health benefits. A mild lipogenic effect was observed for some concentrations of extracts, as well as a slight inhibition in PPAR-alpha expression, although no significant differences were noticeable in the cytokines TNF-alpha, IL-10, and IL-6 as compared with the control. (AU)

• Processo FAPESP: 13/13212-6 - Estudo da aplicação da B-glicosidase de Aspergillus niger LBA 02 imobilizada em lentes PVA-Lentikats® e Sol-Gel na biotransformação de isoflavonas de soja e avaliação dos efeitos biológicos das isoflavonas em modelos de obesidade e inflamação
• Beneficiário: Joelise de Alencar Figueira Angelotti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado