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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genetic Diversity ofXylella fastidiosaPlasmids Assessed by Comparative Genomics

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Autor(es):
Pierry, Paulo Marques [1] ; Uceda-Campos, Guillermo [1, 2] ; Feitosa-Junior, Oseias Rodrigues [1, 2] ; Martins-Junior, Joaquim [1, 2, 3] ; de Santana, Wesley Oliveira [4] ; Della Coletta-Filho, Helvecio [5] ; Zaini, Paulo Adriano [6] ; da-Silva, Aline Maria [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, SP - Brazil
[2] Zaini, Paulo Adriano, Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA.Pierry, Paulo Marques, Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, SP - Brazil
[3] Ctr Nacl Pesquisa Energia & Mat CNPEM, Lab Nacl Biorrenoviveis, BR-13083100 Campinas, SP - Brazil
[4] Univ Estadual Piaui, BR-64260000 Picos, PI - Brazil
[5] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[6] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: TROPICAL PLANT PATHOLOGY; v. 45, n. 3, SI JUN 2020.
Citações Web of Science: 0
Resumo

Plasmids are a major source of horizontal gene transfer among bacteria contributing to their evolution and ecology. The known collection of plasmids carried by the plant pathogenXylella fastidiosaincreases as new strains from different origins and hosts are sampled and sequenced. Here we performed an extensive comparison of 61 publicly available sequences annotated as plasmids belonging to 38X. fastidiosastrains isolated from different plant species and distinct geographical locations. Twenty-six strains exhibited at least one plasmid and up to four plasmids were found in a single strain. Plasmids sizes varied widely from 1.3 kbp to 64.3 kbp, ranging from 1 to 70 protein-coding sequences (CDS) encompassing 324 orthologs. Based on the presence of specific mobility proteins such as relaxases and type 4 secretion system-related genes, respectively 40 and 8 of theX. fastidiosaplasmids were classified as conjugative and mobilizable, while 13 were classified as non-mobilizable.X. fastidiosaplasmids did not carry known antibiotic resistance or virulence genes, and their stability seems to take advantage of toxin/antitoxin systems. The comparative analyses described here revealed similarity among plasmids ofX. fastidiosafrom different subspecies, geographical regions, and hosts, as well as with sequences found in plasmids from other bacterial species. Altogether, our results provide anin silicoanalysis ofX. fastidiosaplasmid content and their main features, with applications in future studies of epidemiology, ecology, and evolution of this phytopathogen. (AU)

Processo FAPESP: 09/13527-1 - Sequenciamento e análise comparativa do genoma de uma cepa do fitopatógeno Xylella fastidiosa.
Beneficiário:Paulo Marques Pierry
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 08/11703-4 - Identificação de genes potencialmente associados adaptação, evolução e virulência de Xylella fastidiosa
Beneficiário:Aline Maria da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/01217-8 - Caracterização da resposta de Citrus sinensis à infecção por Xylella fastidiosa e filogenômica de suas diferentes linhagens.
Beneficiário:Joaquim Martins Junior
Linha de fomento: Bolsas no Brasil - Pós-Doutorado