Development of a system to treat and online monitor photodynamic therapy of skin cancer using PpIX near-infrared fluorescence

Autor(es)

Garcia, Marlon Rodrigues ^{[1, 2]} ; Requena, Michelle Barreto ^[1] ; Pratavieira, Sebastiao ^[1] ; Moriyama, Lilian Tan ^[1] ; Becker, Marcelo ^{[2, 3]} ; Bagnato, Vanderlei Salvador ^{[1, 4, 5]} ; Kurachi, Cristina ^[1] ; Magalhaes, Daniel Varela ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, BR-13566590 Sao Paulo - Brazil

^[3] Bagnato, Vanderlei Salvador, Texas A\&M Univ, Dept Biomed Engn, College Stn, TX USA.Garcia, Marlon Rodrigues, Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, BR-13566590 Sao Paulo - Brazil

^[4] Texas A&M Univ, Hagler Inst Adv Study, College Stn, TX - USA

^[5] Texas A&M Univ, Dept Biomed Engn, College Stn, TX - USA

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 30, JUN 2020.
• Citações Web of Science: 0

Resumo

The limited adoption of photodynamic therapy (PDT) around the medical field may be tied to the unpredicted treatment response that an unmonitored therapy could deliver. Given the high variability in the lesions optical and physiological parameters, it is of fundamental importance to monitor PDT, since different lesions require different therapeutic parameters. We developed a system to treat and online monitor PDT of skin cancer, using protoporphyrin-IX (PpIX) near-infrared fluorescence imaging. The system can be operated up to 150 mW/cm(2) at 633 nm, with real-time fluorescence monitoring around 700 nm, using the treatment light itself for fluorescence excitation. This technology allows system portability, simplicity, and low cost. This study describes the system development and its comparison with a 400-450 nm commercial system to detect the PpIX fluorescence during a PDT in murine skin cancer model. The developed device was able to acquire considerably more fluorescence signal from deeper regions when compared to the violet excitation device. (AU)

• Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático