Gonadal remodeling and hormonal regulation during sex change of juvenile dusky grouperEpinephelus marginatus(Teleostei, Serranidae), an endangered protogynous hermaphrodite fish

Autor(es)

Rodrigues-Filho, J. A. ^{[1, 2]} ; Garcia, C. E. O. ^{[1, 3]} ; Chehade, C. G. ^[4] ; Sanches, E. G. ^[5] ; Borella, I, M. ; Lo Nostro, F. L. ^[6] ; Araujo, B. C. ^{[1, 3]} ; Branco, G. S. ^[7] ; Moreira, R. G. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, Sao Paulo, SP - Brazil

^[2] Fundacao Inst Pesca Estado Rio de Janeiro FIPERJ, Rio De Janeiro - Brazil

^[3] Branco, G. S., Ctr Aquicultura UNESP CAUNESP, Jaboticabal, Brazil.Rodrigues-Filho, J. A., Univ Sao Paulo, Dept Fisiol, Inst Biociencias, Sao Paulo, SP - Brazil

^[4] I, Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Sao Paulo - Brazil

^[5] Inst Pesca APTA SAA, Sao Paulo - Brazil

^[6] Univ Buenos Aires, Inst Biodivers & Biol Expt Aplicada IBBEA, Dept Biodivers & Biol Expt, CONICET, Lab Ecotoxicol Acuat, Fac Ciencias Exactas & Nat, Buenos Aires, DF - Argentina

^[7] Ctr Aquicultura UNESP CAUNESP, Jaboticabal - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FISH PHYSIOLOGY AND BIOCHEMISTRY; v. 46, n. 5 JUN 2020.
• Citações Web of Science: 0

Resumo

Sex change was induced inEpinephelus marginatusjuveniles using a nonsteroidal aromatase inhibitor (AI), a synthetic androgen (17 alpha-methyltestosterone; MT), and a combination of both (MT + AI) in a 90-day experiment. A detailed remodeling of the gonads, the plasma level of gonadal steroids, and immunostaining of pituitary follicle-stimulating hormone (FSH), luteinizing hormone (LH), and somatolactin (SL) cells were analyzed. Sex inversion reached the final spermatogenesis stages using MT, while AI triggered spermatogenesis, but reaching only the spermatid stage. Estradiol (E-2) levels did not change in fish treated with AI but decreased throughout the experimental period in animals treated with MT and MT + AI. Testosterone (T) levels increased in animals treated with MT during the first 60 days (and combined with AI in the first 30 days), decreasing in all experimental groups at 90 days, while AI-treated animals had increased plasma 11-ketotestosterone (11-KT) levels after 90 days. In control fish, FSH- and SL-producing cells (ir-FSH and ir-SL) were restricted to pars intermedia (PI) of the adenohypophysis. Pituitary ir-FSH cells were decreased at the end of the experimental period in all treatments compared with the CT animals. LH-producing cells (ir-LH) were present inproximal pars distalis(PPD) andpars intermedia(PI) of adenohypophysis and did not change after the experimental period. The decreased number of ir-FSH cells at the end of the experiment in all treatments could be related to the negative feedback loop triggered by the increase in natural and/or synthetic androgens. (AU)

• Processo FAPESP: 07/57106-4 - Alterações do tecido gonodal e hipofisário da garoupa Epinephelus marginatus (Teleostei: Serranidae) durante a inversão sexual
• Beneficiário: Jandyr de Almeida Rodrigues Filho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/59122-7 - Inversão sexual e conservação de serranideos: viabilidade na utilização de inibidores de aromatase e monitoramento histofisiologico durante a inversão do sexo da garoupa (Epinephelus marginatus), hermafrodita protogínica
• Beneficiário: Renata Guimarães Moreira Whitton
• Modalidade de apoio: Auxílio à Pesquisa - Regular