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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Polysaccharide-rich hydrogel formulation combined with photobiomodulation repairs UV-induced photodamage in mice skin

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Autor(es):
Grosso Neves, Lia Mara [1] ; Parizotto, Nivaldo Antonio [2, 3, 4] ; Tim, Carla Roberta [2, 3] ; Floriano, Elaine Medeiros [5] ; Vianna Lopez, Renata F. [6] ; Venancio, Tiago [7] ; Fernandes, Joao Batista [7] ; Cominetti, Marcia Regina [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Gerontol, Lab Biol Aging LABEN, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Joint Grad Program Phys Therapy, Sao Carlos, SP - Brazil
[3] Univ Araraquara, Postgrad Program Biotechnol Regenerat Med & Med C, Araraquara, SP - Brazil
[4] Univ Brazil, Postgrad Program Biomed Engn, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Dept Pathol & Legal Med, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeira Preto Sch Pharmaceut Sci FCFRP, Ribeirao Preto, SP - Brazil
[7] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: WOUND REPAIR AND REGENERATION; JUN 2020.
Citações Web of Science: 0
Resumo

Prolonged skin exposure to ultraviolet radiation (UVR) induces premature aging in both the epidermis and the dermis. Chronic exposure to UVR induces the activation of mitogen-activated protein kinase (MAPK) signaling pathway, activating c-Jun, c-Fos expression, and transcription factor of AP-1 activating protein. AP-1 activation results in the positive induction of matrix metalloproteinase (MMP) synthesis, which degrade skin collagen fibers. Polysaccharides from the fruit ofLycium barbarum(LBP fraction) have a range of activities and have been demonstrate to repair the photodamage. In different approaches, laser application aims to recover the aged skin without destroying the epidermis, promoting a modulation, called photobiomodulation (PBM), which leads to protein synthesis and cell proliferation, favoring tissue repair. Here we developed a topical hydrogel formulation from a polysaccharide-rich fraction ofLycium barbarumfruits (LBP). This formulation was associated with PBM (red laser) to evaluate whether the isolated and combined treatments would reduce the UVR-mediated photodamage in mice skin. Hairless mice were photoaged for 6 weeks and then treated singly or in combination with LBP and PBM. Histological, immunohistochemistry, and immunofluorescence analyses were used to investigate the levels of c-Fos, c-Jun, MMP-1, -2, and -9, collagen I, III, and FGF2. The combined regimen inhibited UVR-induced skin thickening, decreased the expression of c-Fos and c-Jun, as well as MMP-1, -2, and -9 and concomitantly increased the levels of collagen I, III, and FGF2. The PBM in combination with LBP treatment is a promising strategy for the repair of photodamaged skin, presenting potential clinical application in skin rejuvenation. (AU)

Processo FAPESP: 13/27021-8 - Efeitos do extrato do fruto de Lycium barbarum L. e do laser vermelho na prevenção e tratamento do fotoenvelhecimento cutâneo em camundongos sem pelos SKH:hr-1
Beneficiário:Lia Mara Grosso Neves
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 15/24940-8 - Avaliação da eficácia de mudanças estruturais do [10]-gingerol em combinação com o quimioterápico doxorubicina para o tratamento de câncer de mama: estudos in vitro e in vivo
Beneficiário:Márcia Regina Cominetti
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras
Beneficiário:João Batista Fernandes
Linha de fomento: Auxílio à Pesquisa - Temático