IL-22 Promotes IFN-gamma-Mediated Immunity against Histoplasma capsulatum Infection

Autor(es):Mostrar menos -

Prado, Morgana K. B. ^{[1, 2, 3]} ; Fontanari, Caroline ^[1] ; Souza, Camila O. S. ^{[1, 3]} ; Gardinassi, Luiz G. ^[1] ; Zoccal, Karina F. ^{[1, 4]} ; de Paula-Silva, Francisco W. G. ^[1] ; Peti, Ana P. F. ^[1] ; Sorgi, Carlos A. ^[1] ; Meirelles, Alyne F. G. ^[1] ; Ramos, Simone G. ^[5] ; Alves-Filho, Jose C. ^[6] ; Faccioli, Lucia H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Goias, Inst Patol Trop & Saude Publ, BR-74605050 Goiania, Go - Brazil

^[3] Univ Sao Paulo, Programa Posgrad Imunol Basica & Aplicada, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Ctr Univ Barao de Maua, BR-14090180 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Dept Patol & Med Legal, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Dept Farmacol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMOLECULES; v. 10, n. 6 JUN 2020.
• Citações Web of Science: 0

Resumo

Histoplasma capsulatum is the agent of histoplasmosis, one of the most frequent mycoses in the world. The infection initiates with fungal spore inhalation, transformation into yeasts in the lungs and establishment of a granulomatous disease, which is characterized by a Th1 response. The production of Th1 signature cytokines, such as IFN-gamma, is crucial for yeast clearance from the lungs, and to prevent dissemination. Recently, it was demonstrated that IL-17, a Th17 signature cytokine, is also important for fungal control, particularly in the absence of Th1 response. IL-22 is another cytokine with multiple functions on host response and disease progression. However, little is known about the role of IL-22 during histoplasmosis. In this study, we demonstrated that absence of IL-22 affected the clearance of yeasts from the lungs and increased the spreading to the spleen. In addition, IL-22 deficient mice (Il22(-/-)) succumbed to infection, which correlated with reductions in the numbers of CD4(+) IFN-gamma(+) T cells, reduced IFN-gamma levels, and diminished nitric oxide synthase type 2 (NOS2) expression in the lungs. Importantly, treatment with rIFN-gamma mitigated the susceptibility of Il22(-/-) mice to H. capsulatum infection. These data indicate that IL-22 is crucial for IFN-gamma/NO production and resistance to experimental histoplasmosis. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/07125-6 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/00658-1 - Novos aspectos funcionais dos eicosanóides
• Beneficiário: Lúcia Helena Faccioli
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários