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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Aminofunctionalized LAPONITE (R) as a versatile hybrid material for chlorhexidine digluconate incorporation: Cytotoxicity and antimicrobial activities

Texto completo
Autor(es):
Peraro, Gustavo R. [1] ; Donzelli, Eduardo H. [1] ; Oliveira, Pollyanna F. [1] ; Tavares, Denise Crispim [1] ; Martins, Carlos H. Gomes [1] ; Molina, Eduardo F. [1] ; de Faria, Emerson H. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Franca, UNIFRAN, Grp Pesquisa Mat Lamelares Hibridos, Ave Dr Armando Salles Oliveira, 201, Parque Univ, BR-14404600 Franca, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: APPLIED CLAY SCIENCE; v. 195, SEP 15 2020.
Citações Web of Science: 0
Resumo

Aminofunctionalized LAPONITE (R); RD was employed for efficient chlorhexidine digluconate (CHX-DG) in-corporation. The structure and properties of the unloaded (Lap-APTES) and loaded (Lap-APTES:CHX-DG) hy-brids were investigated by powder X-ray diffraction (PXRD), thermal analysis, small angle X-ray scattering (SAXS), and infrared absorption spectroscopy (FTIR). A typical adsorption experiment was conducted to evaluate how the parameters contact time and concentration affected CHX-DG incorporation into the clay and showed that this compound had high affinity for Lap-APTES, adsorption equilibrium was reached at 90 min, with CHX-DG incorporation of 1402.7 mg/g. FTIR analysis confirm the interaction by hydrogen bonds between the CHX-DG and APTES amine groups and the laponite OH groups, resulting in materials with potential antibacterial properties for controlled drug release. Even at low concentrations (0.042 mg/g), Lap-APTES:CHX-DG was effective against S. pyogenes. The Lap, Lap-APTES, and Lap-APTES:CHX-DG cytotoxicity to GM07492A cells (human fibroblasts) was investigated by the XTT colorimetric assay, which revealed that CHX-DG incorporation into Lap-APTES reduced drug cytotoxicity. Drug release experiments demonstrated that a maximum of 10% (m/m) CHX-DG was released within 24 h and confirmed the higher affinity between Lap-APTES and CHX-DG. (AU)

Processo FAPESP: 13/19523-3 - Materiais biohíbridos funcionais baseados em argilas lamelares e/ou fibrosas com aplicações multifacetadas
Beneficiário:Emerson Henrique de Faria
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/20455-2 - Materiais híbridos orgânico-inorgânicos nano-estruturados para liberação controlada de lignanas com potencial farmacológico
Beneficiário:Eduardo Ferreira Molina
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 17/15482-1 - Novas estratégias para o reaproveitamento de subprodutos de curtumes para a obtenção de materiais porosos funcionais de alto valor agregado
Beneficiário:Emerson Henrique de Faria
Linha de fomento: Auxílio à Pesquisa - Regular