QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins

Autor(es)

Soratto, Tatiany Aparecida Teixeira ^{[1, 2]} ; Curtolo, Maiara ^{[3, 1]} ; Marengo, Samanta ^[4] ; Dezotti, Ana Lucia ^[1] ; Lima, Romulo Pedro Macedo ^{[1, 5]} ; Gazaffi, Rodrigo ^[6] ; Machado, Marcos Antonio ^[1] ; Cristofani-Yaly, Mariangela ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil

^[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Lab Bioinformat, BR-88040970 Florianopolis, SC - Brazil

^[3] Univ Estadual Campinas, BR-13083862 Campinas, SP - Brazil

^[4] Hill Labs, Hamilton 3204 - New Zealand

^[5] Univ Estado Sao Paulo, Inst Biociencias, Dept Genet, BR-18618000 Botucatu, SP - Brazil

^[6] Univ Fed Sao Carlos, Ctr Ciencias Agr, BR-13600970 Araras, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL PLANT PATHOLOGY; v. 45, n. 6, SI MAY 2020.
• Citações Web of Science: 0

Resumo

Huanglongbing (HLB) is a severe disease of citrus caused by the bacteriumCandidatusLiberibacter. In America, the most common species isCandidatusLiberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization betweenCitrus sunkiHort. Ex Tan. andPoncirus trifoliata(L.) Raf. cv. Rubidoux. The bacterial concentration was higher inC. sunkithan inP. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps ofC. sunkiandP. trifoliatacv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) betweenC. sunkiandP. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in theC. sunkimap and 17 QTL and 40 eQTL in theP. trifoliatamap. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. (AU)

• Processo FAPESP: 16/22133-0 - Edição de genoma visando à validação de eQTLs associados à tolerância a Candidatus Liberibacter asiaticus em citrandarins (Citrus sunki x Poncirus trifoliata)
• Beneficiário: Maiara Curtolo
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
• Beneficiário: Marcos Antonio Machado
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/18605-0 - Obtenção e avaliação de novas variedades copas e porta-enxertos para citricultura de mesa
• Beneficiário: Mariângela Cristofani-Yaly
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/00133-4 - Ensaios com citrandarins como porta-enxertos para as laranjas Pêra e Valência e a lima ácida Tahiti
• Beneficiário: Mariângela Cristofani-Yaly
• Linha de fomento: Auxílio à Pesquisa - Regular