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A comparative pan-genomic analysis of 53C. pseudotuberculosisstrains based on functional domains

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Bernardes, Juliana S. [1] ; Eberle, Raphael J. [2] ; Vieira, Fabio R. J. [3] ; Coronado, Monika A. [2, 4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Sorbonne Univ, CNRS, UMR 7238, Lab Biol Computat & Quantitat, Paris - France
[2] Univ Estadual Paulista UNESP, Inst Biociencias Letras & Ciencias Exatas Ibilce, Dept Phys, Multiuser Ctr Biomol Innovat, Sao Jose Do Rio Preto - Brazil
[3] Inst Biol Ecole Normale Super IBENS, Paris - France
[4] Forschungszentrum Juelich, Inst Biol Informat Proc IBI 7 Strucut Biochem, Wilhelm Johnen Str, Julich - Germany
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Citações Web of Science: 0

Corynebacterium pseudotuberculosisis a pathogenic bacterium with great veterinary and economic importance. It is classified into two biovars:ovis, nitrate-negative, that causes lymphadenitis in small ruminants andequi, nitrate-positive, causing ulcerative lymphangitis in equines. With the explosive growth of available genomes of several strains, pan-genome analysis has opened new opportunities for understanding the dynamics and evolution ofC. pseudotuberculosis. However, few pan-genomic studies have compared biovarsequiandovis.Such studies have considered a reduced number of strains and compared entire genomes. Here we conducted an original pan-genome analysis based on protein sequences and their functional domains. We considered 53 C. pseudotuberculosisstrains from both biovars isolated from different hosts and countries. We have analysed conserved domains, common domains more frequently found in each biovar and biovar-specific (unique) domains. Our results demonstrated that biovarequiis more variable; there is a significant difference in the number of proteins per strains, probably indicating the occurrence of more gene loss/gain events. Moreover, strains of biovarequipresented a higher number of biovar-specific domains, 77 against only eight in biovarovis, most of them are associated with virulence mechanisms. With this domain analysis, we have identified functional differences among strains of biovarsovisandequithat could be related to niche-adaptation and probably help to better understanding mechanisms of virulence and pathogenesis. The distribution patterns of functional domains identified in this work might have impacts on bacterial physiology and lifestyle, encouraging the development of new diagnoses, vaccines, and treatments forC. pseudotuberculosisdiseases. Communicated by Ramaswamy H. Sarma (AU)

Processo FAPESP: 16/12904-0 - Mecanismos e Interações Moleculares de Moléculas Bioativas com a Protease NS3 do Zika Vírus.
Beneficiário:Monika Aparecida Coronado
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/07572-3 - Explorando a protease nsP2 dos vírus Chikungunya e Mayaro: estruturas e inibição.
Beneficiário:Raphael Josef Eberle
Linha de fomento: Bolsas no Brasil - Pós-Doutorado