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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination

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Autor(es):
Kassab, Giulia [1] ; Cheburkanov, Vsevolod [2] ; Willis, Jace [2] ; Moule, Madeleine G. [3] ; Kurachi, Cristina [1] ; Yakovlev, Vladislav [2] ; Cirillo, Jeffrey D. [3] ; Bagnato, Vanderlei S. [1, 4]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos - Brazil
[2] Texas A&M Univ, Dept Biomed Engn, College Stn, TX - USA
[3] Texas A&M Univ, Coll Med, Dept Microbial Pathogenesis & Immunol, Bryan, TX - USA
[4] Texas A&M Univ, Hagler Inst Adv Studies, College Stn, TX - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Biophotonics; AUG 2020.
Citações Web of Science: 0
Resumo

Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing ofStaphylococcus aureus(of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused byS. aureusand other important pathogens. (AU)

Processo FAPESP: 18/18188-0 - Inativação fotodinâmica da Pneumonia Bacteriana utilizando nebulização do fotossensibilizador e iluminação extracorpórea
Beneficiário:Giulia Kassab
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs