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Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study

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Nascimento, Glauce C. [1] ; Malzone, Bruno L. [1] ; Iyomasa, Daniela M. [2] ; Pereira, Yamba C. L. [1] ; Issa, Joao Paulo M. [1] ; Leite-Panissi, Christie R. A. [1] ; Watanabe, Ii-Sei [3] ; Iyomasa, Mamie M. [1] ; Fuentes, Ramon [4] ; Del Bel, Elaine [1] ; Dias, Fernando J. [4]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Ribeirao Preto, SP - Brazil
[2] Univ Oeste Paulista, Presidente Prudente Med Sch, Dept Morphol, Presidente Prudente, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo, SP - Brazil
[4] Univ La Frontera, Res Ctr Dent Sci CICO, Dent Sch, Dept Integral Dent, Temuco - Chile
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAY 29 2020.
Citações Web of Science: 0

Psychological stress and occlusal alteration are important etiologic factors for temporomandibular/masticatory muscular disorders. In particular, the exact physiologic mechanism underlying the relation by occlusal alteration and temporomandibular disorders remains unclear. Our purpose was to test the hypothesis that benzodiazepine therapy is able to prevent metabolic and vascular changes in the medial pterygoid muscle of rats under chronic stress after 14 days of unilateral exodontia. Adult Wistar rats were submitted to unpredictable chronic mild stress (10 days) and/or unilateral exodontia and their plasma and medial pterygoid muscles were removed for analysis. A pre-treatment with diazepam was used to verify its effect on stress. The parameters evaluated included anxiety behavior, plasma levels of corticosterone, metabolic activity by succinate dehydrogenase, capillary density by laminin staining and ultrastructural findings by transmission electron microscopy. Occlusal instability induced anxiety-like behavior on elevated plus-maze test and diazepam administration blocked the appearance of this behavior. Unilateral exodontia promoted in the contralateral muscle an increase of oxidative fibers and capillaries and modification of sarcoplasmic reticulum. Chronic stress caused increased glycolytic metabolism, reduced capillary density and morphological changes in mitochondria on both sides. Association of both factors induced a glycolytic pattern in muscle and hemodynamic changes. Pharmacological manipulation with diazepam inhibited the changes in the medial pterygoid muscle after stress. Our results reveal a preventive benzodiazepine treatment for stress and occlusal instability conditions affecting masticatory muscle disorders. In addition, provide insights into the mechanisms by which chronic stress and exodontia might be involved in the pathophysiology of masticatory muscular dysfunctions. (AU)

Processo FAPESP: 12/22128-6 - Efeitos do estresse sistêmico associado à maloclusão sobre o músculo pterigóideo medial e encéfalo
Beneficiário:Mamie Mizusaki Iyomasa
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/03053-3 - Avaliação de músculo pterigóideo medial e estruturas encefálicas relacionadas à nocicepção orofacial e comportamento emocional em modelo animal de estresse crônico e hipofunção mastigatória
Beneficiário:Glauce Crivelaro Do Nascimento
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado