Impact of Dry Heating in an Alkaline Environment on the Structure and Foaming Properties of Whey Proteins

Autor(es)

Vidotto, Danilo C. ^[1] ; Tavares, Guilherme M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Bioprocess Technology; v. 13, n. 10 AUG 2020.
• Citações Web of Science: 0

Resumo

Dry heating (DH) in an alkaline environment has been proposed as a treatment that can improve some techno-functional properties of whey proteins, such as their water-holding capacity and gelling properties. Nevertheless, information concerning the impact of DH in an alkaline environment on the foaming properties of whey proteins is not available in the literature. In this context, lyophilized whey protein isolate (WPI) powders with and without added lactose were submitted to DH treatments (60 and 80 degrees C) under neutral and alkaline environments for 48 h. Even without DH, the alkaline environment induced the formation of insoluble protein aggregates stabilized by disulfide and non-disulfide covalent interactions. The amount of insoluble protein aggregates enhanced with the increase in the intensity of DH. No insoluble aggregates were observed for samples produced in a neutral environment. Furthermore, no difference in the apparent secondary structure of the proteins in the soluble fraction of neutral or alkaline equivalent samples could be evidenced. In addition, the intensity of the population of soluble protein aggregates rose by increasing the intensity of DH treatment; however, it was comparable between neutral and alkaline equivalent samples. DH in an alkaline environment at 80 degrees C for 48 h significantly enhanced the stability of the foams produced by the soluble fraction of lactose-added samples, corroborating its potential to improve useful techno-functional properties of whey proteins. (AU)

• Processo FAPESP: 17/09214-4 - Como modificações estruturais de proteínas lácteas afetam a funcionalidade de compostos bioativos em matrizes alimentares complexas?
• Beneficiário: Guilherme Miranda Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/09304-6 - Interação entre carotenoides e proteínas lácteas globulares nativas e não nativas: impactos estruturais e funcionais em sistemas mono e multi protéicos
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• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/03822-5 - EMU concedido no processo 2017/09214-4: cromatógrafo preparativo - Akta pure (GE healthcare)
• Beneficiário: Guilherme Miranda Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários