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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Exploring the interaction between citrus flavonoids and phytopathogenic fungi through enzymatic activities

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Autor(es):
Costa, Jonas Henrique [1] ; Fernandes, Laura Soler [1] ; Akiyama, Daniel Yuri [1] ; Fill, Taicia Pacheco [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: BIOORGANIC CHEMISTRY; v. 102, SEP 2020.
Citações Web of Science: 0
Resumo

Flavonoids are involved in citrus defense against phytopathogens. In this study, we applied in vitro biocatalysis assays using the flavanones glycosides hesperidin and naringin to explore the enzymatic activities involved in such interaction. The main enzymatic activity observed was the hydrolysis catalyzed by fungi naringinases and hesperidinases. Withing 7 days, the two citrus phytopathogenic fungi, Penicillium digitatum and Penicillium italicum, exhibited the highest hydrolyzing rate on the flavanones, reaching conversion values higher than 90%. In addition, Geothrichum citri-aurantii exhibited no enzymatic activity and Penicillium expansum only hydrolyzed hesperidin. In order to evaluate flavonoid biotransformation by the fungi in vivo, citrus fruits infected with P. digitatum were analyzed through molecular networking and Imaging Mass Spectrometry (IMS). In vivo assays revealed that citrus fruit in response to the infection is able to hydroxylate flavonoids, and novel flavonoid structures were associated to the citrus' defense. The data reported here present a new point of view in the relation between citrus flavonoids and phytopathogenic fungi and can be useful to understand the infection processes and host-pathogen interaction. (AU)

Processo FAPESP: 18/13027-8 - Análise do perfil metabólico pré e pós-deleção do gene histona deacetilase no fungo Penicillium brasilianum
Beneficiário:Daniel Yuri Akiyama
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/24462-4 - Entendendo as estratégias de virulência do fungo Penicillium digitam contra seu hospedeiro citros
Beneficiário:Taicia Pacheco Fill
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 19/06359-7 - Compreensão dos fatores químicos envolvidos no endofitismo x patogenicidade de diferentes isolados de Penicillium brasilianum
Beneficiário:Taicia Pacheco Fill
Modalidade de apoio: Auxílio à Pesquisa - Regular