Chitosan hydrogel covalently crosslinked by gold nanoparticle: Eliminating the use of toxic crosslinkers

Autor(es)

Tenorio, Fernanda Silva ^[1] ; do Amaral Montanheiro, Thais Larissa ^[2] ; Isaias dos Santos, Alexandre Martins ^[1] ; Silva, Mateus dos Santos ^[3] ; Lemes, Ana Paula ^[3] ; Tada, Dayane Batista ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Lab Nanomat & Nanotoxicol, Talim 330, BR-12231280 Sao Jose Dos Campos, SP - Brazil

^[2] Inst Tecnol Aeronaut, Lab Plasmas & Proc, Sao Jose Dos Campos, SP - Brazil

^[3] Univ Fed Sao Paulo, Polymer & Biopolymer Technol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Polymer Science; v. 138, n. 6 AUG 2020.
• Citações Web of Science: 0

Resumo

Tissue engineering has directed a lot of effort toward the development of devices with suitable biocompatibility and mechanical properties. Chitosan has been pointed as a valuable material to be applied in scaffolds due to its antimicrobial activity and biocompatibility. Nevertheless, the low mechanical resistance associated with the requirement of toxic crosslinkers has hampered translational application of chitosan hydrogel. Herein, the use of gold nanoparticles (AuNP) as crosslinker is reported as a great strategy to obtain chitosan hydrogel without using toxic reactants. In addition, the resultant chitosan hydrogel, crosslinked by AuNP of 30 nm (AuNP30), presented outstanding properties compared to chitosan hydrogel crosslinked by glutaraldehyde. Chitosan hydrogel crosslinked by AuNP30 presented lower porosity, which provided lower swelling degree and slower degradation rate. In addition, compressive strength was about two times higher than the chitosan hydrogel crosslinked by glutaraldehyde. The crosslink by AuNP30 also increased the biocompatibility of the hydrogel. Chitosan hydrogel crosslinked by AuNP30 did not show cytotoxicity against MEF cells, whereas cell viability of cells incubated with extract from chitosan hydrogel crosslinked by glutaraldehyde was only 41%. In conclusion, the results reported herein pointed that the use of AuNP30 as crosslinker agent provided to chitosan hydrogel enhanced properties that made it suitable to application in biomedical devices. (AU)

• Processo FAPESP: 17/01697-6 - Síntese e funcionalização de nanopartículas bimetálicas para aplicação na terapia fotodinâmica de câncer
• Beneficiário: Dayane Batista Tada
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/24873-4 - Nanocompósito de PHBV e NTC funcionalizado com hidroxibutirato: estudo cinético da cristalização e correlação com as propriedades mecânicas
• Beneficiário: Thaís Larissa do Amaral Montanheiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/27064-9 - Utilização de nanocristais de celulose na produção de nanocompósitos poliméricos para aplicações na área médica
• Beneficiário: Ana Paula Lemes
• Modalidade de apoio: Auxílio à Pesquisa - Regular