Development and evaluation of reparative tricalciumsilicate-ZrO2-Biosilicatecomposites

Autor(es)

Queiroz, Marcela B. ^[1] ; Torres, Fernanda F. E. ^[1] ; Rodrigues, Elisandra M. ^[1] ; Viola, Kennia S. ^[1] ; Bosso-Martelo, Roberta ^[2] ; Chavez-Andrade, Gisselle M. ^[1] ; Souza, Marina T. ^[3] ; Zanotto, Edgar D. ^[3] ; Guerreiro-Tanomaru, Juliane M. ^[1] ; Tanomaru-Filho, Mario ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Araraquara, SP - Brazil

^[2] Bahia Fed Univ UFBA, Sch Dent, Dept Dent, Salvador, BA - Brazil

^[3] Fed Univ Sao Carlos UFSCar, CeRTEV Ctr Res Technol & Educ Vitreous Mat, Dept Mat Engn, Vitreous Mat Lab LaMaV, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 109, n. 4 AUG 2020.
• Citações Web of Science: 0

Resumo

Biosilicate is a bioactive glass-ceramic used in medical and dental applications. This study evaluated novel reparative materials composed of pure tricalcium silicate (TCS), 30% zirconium oxide (ZrO2) and 10 or 20% biosilicate, in comparison with Biodentine. Setting time was evaluated based on ISO 6876 standard, radiopacity by radiographic analysis, solubility by mass loss, and pH by using a pH meter. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and NR assays. Alkaline phosphatase (ALP) activity and alizarin red were used to evaluate cell bioactivity. Antimicrobial activity was assessed onEnterococcus faecalisby the direct contact test. The data were submitted to analysis of variance (ANOVA)/Tukey; Bonferroni and Kruskal-Wallis, and Dunn tests (alpha= 0.05). The association of Biosilicate with TCS + ZrO(2)had appropriate setting time, radiopacity, and solubility, alkaline pH, and antimicrobial activity. TCS and Biodentine showed higher ALP activity in 14 days than the control (serum-free medium). All cements produced mineralized nodules. In conclusion, Biosilicate + TCS ZrO(2)decreased the setting time and increased the radiopacity in comparison to TCS. Biosilicate + TCS ZrO2 presented lower solubility and higher radiopacity than Biodentine. In addition, these experimental cements promoted antimicrobial activity and mineralization nodules formation, suggesting their potential for clinical use. (AU)

• Processo FAPESP: 17/19049-0 - Avaliação das etapas do tratamento de canais radiculares, e das propriedades físico-químicas e biológicas de materiais à base de silicato tricálcico
• Beneficiário: Mario Tanomaru Filho
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03437-6 - Avaliação do preparo reciprocante, da obturação dos canais radiculares, e das propriedades físico-químicas e biológicas de materiais obturadores e reparadores
• Beneficiário: Mario Tanomaru Filho
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/00321-0 - Desenvolvimento de novas metodologias utilizando micro-CT para avaliação de propriedades físico-químicas de materiais reparadores e cimentos endodônticos
• Beneficiário: Fernanda Ferrari Esteves Torres
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/11036-4 - Características físico-químicas e biológicas de novos materiais reparadores à base de silicato tricálcico e biosilicato
• Beneficiário: Marcela Borsatto Queiroz
• Linha de fomento: Bolsas no Brasil - Mestrado