Laser microdissection-based microproteomics of the hippocampus of a rat epilepsy model reveals regional differences in protein abundances

Autor(es):Mostrar menos -

Canto, Amanda M. ^{[1, 2]} ; Vieira, Andre S. ^{[2, 3]} ; Matos, Alexandre H. B. ^{[1, 2]} ; Carvalho, Benilton S. ^{[2, 4]} ; Henning, Barbara ^{[1, 2]} ; Norwood, Braxton A. ^{[5, 6]} ; Bauer, Sebastian ^{[6, 7]} ; Rosenow, Felix ^{[6, 7]} ; Gilioli, Rovilson ^[8] ; Cendes, Fernando ^{[2, 9]} ; Lopes-Cendes, Iscia ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Med Sci, Dept Med Genet & Genom Med, UNICAMP, Campinas - Brazil

^[2] Brazilian Inst Neurosci & Neurotechnol BRAINN, Campinas - Brazil

^[3] Univ Estadual Campinas, Biol Inst, Dept Funct & Struct Biol, UNICAMP, Campinas - Brazil

^[4] Univ Estadual Campinas, Dept Stat, Inst Math Stat & Sci Comp, UNICAMP, Campinas - Brazil

^[5] Expesicor Inc, Kalispell, MT - USA

^[6] Philipps Univ Marburg, Epilepsy Ctr Hessen, Dept Neurol, Marburg - Germany

^[7] Goethe Univ Frankfurt, Epilepsy Ctr Frankfurt Rhine Main, Frankfurt - Germany

^[8] Univ Estadual Campinas, Lab Anim Qual Control, UNICAMP, Campinas - Brazil

^[9] Univ Estadual Campinas, Sch Med Sci, Dept Neurol, UNICAMP, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAR 10 2020.
• Citações Web of Science: 0

Resumo

Mesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder affecting almost 40% of adult patients with epilepsy. Hippocampal sclerosis (HS) is a common histopathological abnormality found in patients with MTLE. HS is characterised by extensive neuronal loss in different hippocampus subregions. In this study, we used laser microdissection-based microproteomics to determine the protein abundances in different regions and layers of the hippocampus dentate gyrus (DG) in an electric stimulation rodent model which displays classical HS damage similar to that found in patients with MTLE. Our results indicate that there are differences in the proteomic profiles of different layers (granule cell and molecular), as well as different regions, of the DG (ventral and dorsal). We have identified new signalling pathways and proteins present in specific layers and regions of the DG, such as PARK7, RACK1, and connexin 31/gap junction. We also found two major signalling pathways that are common to all layers and regions: inflammation and energy metabolism. Finally, our results highlight the utility of high-throughput microproteomics and spatial-limited isolation of tissues in the study of complex disorders to fully appreciate the large biological heterogeneity present in different cell populations within the central nervous system. (AU)

• Processo FAPESP: 13/07559-3 - Instituto Brasileiro de Neurociência e Neurotecnologia - BRAINN
• Beneficiário: Fernando Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/08635-5 - Análise do transcriptoma em modelos animais de epilepsia do lobo temporal mesial
• Beneficiário: Alexandre Hilário Berenguer de Matos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/12960-4 - Análise proteômica de tecido hipocampal obtido de pacientes e de modelos animais de Epilepsia do Lobo Temporal Mesial: um estudo comparativo
• Beneficiário: Amanda Morato Do Canto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/19484-6 - Análise proteômica quantitativa de tecido neuronal obtido de pacientes e modelos animais de epilepsia
• Beneficiário: Barbara Henning Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado