Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression

Autor(es)

Bernardes, Willian Souza ^[1] ; Menossi, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Funct Genome Lab, Dept Genet Evolut Microbiol & Immunol, Inst Biol, Campinas - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 11, AUG 14 2020.
• Citações Web of Science: 1

Resumo

Molecular biotechnology has made it possible to explore the potential of plants for different purposes. The 3' regulatory regions have a great diversity ofcis-regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. A complex interaction between the cleavage and polyadenylation molecular complex andcis-elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. In addition, after transcription, a wide array of RNA-binding proteins interacts withcis-acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such asNOSandOCSfromAgrobacterium tumefaciensand35Sfrom cauliflower mosaic virus. In this review, we discuss the role of 3' regulatory regions in gene expression, and the superior potential that plant 3' regulatory regions have compared toNOS, OCSand35S3' regulatory regions. (AU)

• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/15576-5 - Abordagens moleculares para estudar a relação entre etileno e maturação em cana-de-açúcar
• Beneficiário: Marcelo Menossi Teixeira
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático