| Texto completo | |
| Autor(es): |
Betancur, Luz A.
[1, 2]
;
Forero, Abel M.
[1]
;
Vinchira-Villarraga, Diana M.
[1]
;
Cardenas, Juan D.
[1]
;
Romero-Otero, Adriana
[1]
;
Chagas, Fernanda O.
[3, 4]
;
Pupo, Monica T.
[3]
;
Castellanos, Leonardo
[1]
;
Ramos, Freddy A.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Nacl Colombia, Dept Quim, Sede Bogota, Carrera 30 45-03, Edificio Quim 427, Bogota 111321 - Colombia
[2] Univ Caldas, Dept Quim, Edificio Orlando Sierra, Bloque B, Manizales, Caldas - Colombia
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Fed Univ Rio de Janeiro IPPN UFRJ, Walter Mors Inst Res Nat Prod, Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROBIOLOGICAL RESEARCH; v. 239, OCT 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites' production of Streptomyces sp. PNM-9 from the algae Dictyota sp. and their biological activity against the rice phytopathogenic bacteria Burkholderia spp. The compounds 2-methyl-N-(2 `-phenylethyl)-butanamide (1) and 3-methyl-N-(2 `-phenylethyl)-butanamide (2) were identified through MVDA and 2D NMR experiments in the organic extract of a 15-days LB media culture of Streptomyces sp. PNM-9. Compounds 1 and 2 were isolated and their structures confirmed by oneand two-dimensional NMR and mass spectrometry (MS) data. Compounds 1 and 2 were active against the rice pathogenic bacteria Burkholderia glumae (ATCC 33,617) displaying minimal inhibitory concentration (MIC) values of 2.43 mM and 1.21 mM, respectively. The metabolomics-guided approach employing NMR-metabolic profiling was useful for marine microbial bioprospecting and suggested Streptomyces sp. PNM-9 strain and its compounds as a potential control against phytopathogenic bacteria. (AU) | |
| Processo FAPESP: | 13/50954-0 - Novos agentes terapêuticos obtidos de bactérias simbiontes de invertebrados brasileiros |
| Beneficiário: | Mônica Tallarico Pupo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |