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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Neurochemical phenotype of growth hormone-responsive cells in the mouse paraventricular nucleus of the hypothalamus

Texto completo
Autor(es):
Quaresma, Paula G. F. [1] ; dos Santos, Willian O. [1] ; Wasinski, Frederick [1] ; Metzger, Martin [1] ; Donato Jr, Jose
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF COMPARATIVE NEUROLOGY; v. 529, n. 6 SEP 2020.
Citações Web of Science: 2
Resumo

Multiple neuroendocrine, autonomic and behavioral responses are regulated by the paraventricular nucleus of the hypothalamus (PVH). Previous studies have shown that PVH neurons express the growth hormone (GH) receptor (GHR), although the role of GH signaling on PVH neurons is still unknown. Given the great heterogeneity of cell types located in the PVH, we performed a detailed analysis of the neurochemical identity of GH-responsive cells to understand the possible physiological importance of GH action on PVH neurons. GH-responsive cells were detected via the phosphorylated form of the signal transducer and activator of transcription-5 (pSTAT5) in adult male mice that received an intraperitoneal GH injection. Approximately 51% of GH-responsive cells in the PVH co-localized with the vesicular glutamate transporter 2. Rare co-localizations between pSTAT5 and vesicular GABA transporter or vasopressin were observed, whereas approximately 20% and 38% of oxytocin and tyrosine hydroxylase (TH) cells, respectively, were responsive to GH in the PVH. Approximately 55%, 35% and 63% of somatostatin, thyrotropin-releasing hormone (TRH) and corticotropin-releasing hormone (CRH) neurons expressed GH-induced pSTAT5, respectively. Additionally, 8%, 49% and 75% of neuroendocrine TH, TRH and CRH neurons, and 67%, 32% and 74% of nonneuroendocrine TH, TRH and CRH neurons were responsive to GH in the PVH of Fluoro-Gold-injected mice. Our findings suggest that GH action on PVH neurons is involved in the regulation of the thyroid, somatotropic and adrenal endocrine axes, possibly influencing homeostatic and stress responses. (AU)

Processo FAPESP: 16/20897-3 - Papel dos neurônios orexina como mediadores dos efeitos centrais induzidos pelo hormônio do crescimento
Beneficiário:Frederick Wasinski
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/25281-3 - Efeitos do hormônio do crescimento sobre os neurônios POMC, colinérgicos e do núcleo paraventricular do hipotálamo: implicações no controle do metabolismo energético e glicêmico
Beneficiário:Paula Gabriele Fernandes Quaresma Bergonsi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/16473-6 - Processamento de estímulos aversivos: circuitaria entre o núcleo tegmental laterodorsal, a habênula, e os núcleos dorsal e mediano da rafe
Beneficiário:Martin Andreas Metzger
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/02983-2 - Ação do hormônio do crescimento no sistema nervoso: relevância para as funções neurais e na doença
Beneficiário:Jose Donato Junior
Linha de fomento: Auxílio à Pesquisa - Temático