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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Capsaicin lacks tumor-promoting effects during colon carcinogenesis in a rat model induced by 1,2-dimethylhydrazine

Texto completo
Autor(es):
Ramos Caetano, Brunno Felipe [1] ; Tablas, Mariana Baptista [2] ; Ignoti, Marcela Goncalves [2] ; de Moura, Nelci Antunes [2] ; Romualdo, Guilherme Ribeiro [2] ; Barbisan, Luis Fernando [2] ; Marchesan Rodrigues, Maria Aparecida [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Med Sch, Dept Pathol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Biosci Inst, Dept Struct & Funct Biol, BR-18618689 Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Environmental Science and Pollution Research; v. 28, n. 2 SEP 2020.
Citações Web of Science: 1
Resumo

Capsaicin (CPS, 8-methyl-N-vanillyl-trans-6-nonenamide), a pungent alkaloid from chili peppers, has contradictory effects in both experimental and human carcinogenesis. Thus, we evaluated the modifying effects of chronic CPS during the promotion and progression stages of rat colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH). Male Wistar rats were given four subcutaneous injections of DMH (40 mg/body weight (b.w.)) twice a week, for 2 weeks. After DMH-induced tumor initiation, the animals were treated with CPS at 5 or 50 mg/kg b.w. by gavage for 24 weeks (three times a week). High-dose CPS reduced both cell proliferation in adjacent ``normal-appearing{''} colonic crypts and the total number of preneoplastic aberrant crypt foci (ACF) but did not change the number of dysplastic ACF or ACF multiplicity. Although the proportion of adenomas was increased, and tubular adenocarcinomas decreased in high-dose CPS, both CPS interventions exerted no effects on total tumor incidence, volume, multiplicity, cell proliferation (Ki-67), and apoptosis (caspase-3). In accordance, high-dose CPS treatment had discrete effects on gene expression in colon tumors, as only 3/94 (3.19%) genes were significantly modified (downregulation ofCebpdandFasl, and upregulation ofJag1). The findings of the present study show that CPS does not impact on the promotion/progression stages of rat colon carcinogenesis. Therefore, CPS at a high-dose intervention showed to be a safe food ingredient. (AU)

Processo FAPESP: 14/24762-0 - Quimioprevenção do câncer do cólon pela capsaicina: influência nas etapas de iniciação e promoção em modelo de carcinogênese do cólon pela dimetilhidrazina no rato
Beneficiário:Maria Aparecida Marchesan Rodrigues Kobayasi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/21951-6 - Efeitos da capsaicina na etapa de iniciação da carcinogênese química de colón em ratos
Beneficiário:Brunno Felipe Ramos Caetano
Linha de fomento: Bolsas no Brasil - Mestrado