Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Novel Borrelia genotypes in bats from the Macaregua Cave, Colombia

Texto completo
Autor(es):
Munoz-Leal, Sebastian [1, 2] ; Faccini-Martinez, Alvaro A. [3, 4] ; Perez-Torres, Jairo [5] ; Chala-Quintero, Sandra M. [5] ; Herrera-Sepulveda, Maria T. [5, 6] ; Cuervo, Claudia [6] ; Labruna, Marcelo B. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Vet Med & Zootechny, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil
[2] Univ Concepcion, Dept Pathol & Prevent Med, Fac Vet Sci, Ave Vicente Mendez 595, Casilla 537, Chillan, Nuble - Chile
[3] Univ Cordoba, Inst Invest Biol Trop, Campus Berastegui, Cordoba - Colombia
[4] Asociac Colombiana Infectol, Comm Trop Med Zoonoses & Travel Med, Bogota - Colombia
[5] Pontificia Univ Javeriana, Ecol & Systemat Unit UNESIS, Lab Funct Ecol, Fac Sci, Bogota - Colombia
[6] Pontificia Univ Javeriana, Infect Dis Grp, Fac Sci, Bogota - Colombia
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ZOONOSES AND PUBLIC HEALTH; v. 68, n. 1 NOV 2020.
Citações Web of Science: 0
Resumo

Bats have been implicated as reservoirs of relapsing fever group spirochaetes since the beginning of the last century. Recently, bat-associated spirochaetes have been reported as human pathogens. In 1968, a spirochaete was detected in blood of the bat Natalus tumidirostris captured inside the Macaregua cave, Colombia. Data on this microorganism were never published again. The aim of this study was to evaluate the presence of Borrelia DNA in blood from bats of Macaregua cave. We performed molecular analyses using a genus-specific real-time PCR targeting the 16S rRNA to detect DNA of Borrelia in blood samples from 46 bats captured in the Macaregua cave. Positive samples were submitted to a battery of PCRs aiming to amply Borrelia 16S rRNA, flaB, glpQ, p66, ospC, clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA genes. Seventeen samples were positive for Borrelia after real-time PCR. With the exception of flaB gene, attempts to amplify further loci were unsuccessful. Nucleotide and amino acid divergences of four flaB haplotypes characterized from blood of Carollia perspicillata showed Borrelia burgdorferi sensu lato (Bbsl) as the most closely related group. A phylogenetic tree including 74 sequences of the genus confirmed this trend, since Borrelia genotypes detected in bats from Macaregua formed a monophyletic group basally positioned to Bbsl. Our results suggest that Borrelia genotypes characterized from bats roosting in the Macaregua cave might constitute a new taxon within the genus. This is the first molecular characterization of a Borrelia sp. in Colombia. (AU)

Processo FAPESP: 18/02521-1 - Pesquisa de espiroquetas do grupo da febre recorrente (Spirochaetaceae: Borrelia) em carrapatos do gênero Ornithodoros (Acari: Argasidae) parasitas de humanos no Brasil
Beneficiário:Sebastián Alejandro Munoz Leal
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 19/17960-3 - Técnicas de cultivo avançadas, sequenciamento de última geração e inmunoblot aplicado ao estudo de uma espiroqueta do grupo da febre recorrente brasileira
Beneficiário:Sebastián Alejandro Munoz Leal
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado