Avoiding ventilator-associated pneumonia: Curcumin-functionalized endotracheal tube and photodynamic action

Autor(es)

Zangirolami, Amanda C. ^[1] ; Dias, Lucas D. ^[1] ; Blanco, Kate C. ^[1] ; Vinagreiro, Carolina S. ^[2] ; Inada, Natalia M. ^[1] ; Arnaut, Luis G. ^[2] ; Pereira, Mariette M. ^[2] ; Bagnato, Vanderlei S. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Coimbra, Coimbra Chem Ctr, Dept Chem, P-3004535 Coimbra - Portugal

^[3] Texas A&M Univ, Hagler Inst Adv Studies, College Stn, TX 77843 - USA

• Tipo de documento: Artigo Científico
• Fonte: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 117, n. 37, p. 22967-22973, SEP 15 2020.
• Citações Web of Science: 0

Resumo

Hospital-acquired infections are a global health problem that threatens patients' treatment in intensive care units, causing thousands of deaths and a considerable increase in hospitalization costs. The endotracheal tube (ETT) is a medical device placed in the patient's trachea to assist breathing and delivering oxygen into the lungs. However, bacterial biofilms forming at the surface of the ETT and the development of multidrug-resistant bacteria are considered the primary causes of ventilator-associated pneumonia (VAP), a severe hospital-acquired infection for significant mortality. Under these circumstances, there has been a need to administrate antibiotics together. Although necessary, it has led to a rapid increase in bacterial resistance to antibiotics. Therefore, it becomes necessary to develop alternatives to prevent and combat these bacterial infections. One possibility is to turn the ETT itself into a bactericide. Some examples reported in the literature present drawbacks. To overcome those issues, we have designed a photosensitizer-containing ETT to be used in photodynamic inactivation (PDI) to avoid bacteria biofilm formation and prevent VAP occurrence during tracheal intubation. This work describes ETT's functionalization with curcumin photosensitizer, as well as its evaluation in PDI against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. A significant photoinactivation (up to 95%) against Gram-negative and Gram-positive bacteria was observed when curcumin-functionalized endotracheal (ETT-curc) was used. These remarkable results demonstrate this strategy's potential to combat hospital-acquired infections and contribute to fighting antimicrobial resistance. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/13569-8 - Estudo dos Mecanismos de Ação em Terapia Fotodinâmica: Do Fotossensibilizador à Aplicação Prática
• Beneficiário: Lucas Danilo Dias
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/12694-3 - Tratamento de faringotonsilites com ação fotodinâmica
• Beneficiário: Kate Cristina Blanco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado