Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation

Autor(es)

Freitas, Gileade P. ^[1] ; Souza, Alann T. P. ^[1] ; Lopes, Helena B. ^[1] ; Trevisan, Rayana L. B. ^[1] ; Oliveira, Fabiola S. ^[1] ; Fernandes, Roger R. ^[1] ; Ferreira, Fernanda U. ^[2] ; Rose, Felipe A. ^[2] ; Beloti, Marcio M. ^[1] ; Rosa, Adalberto L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Hemotherapy Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIO-PROTOCOL; v. 10, n. 4 FEB 20 2020.
• Citações Web of Science: 1

Resumo

Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of abdominal and inguinal adipose tissues, MSCs are selected by their adherence to the plastic tissue culture dish. Within 7 days, MSCs reach 70% confluence and are ready to be used in subsequent experiments. The protocols described here are easy to perform, cost-efficient, require minimal time, and yield a cell population rich in MSCs. (AU)

• Processo FAPESP: 18/17356-6 - Efeito da Superfície de Titânio com Nanotopografia sobre a Interação entre Osteoblastos e Osteoclastos
• Beneficiário: Márcio Mateus Beloti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/12622-7 - Terapia celular: potencial de células-tronco mesenquimais, VEGF-A e BMP-9 para regenerar tecido ósseo
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Temático