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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Insights in Osteosarcoma by Proton Nuclear Magnetic Resonance Serum Metabonomics

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Autor(es):
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Quintero Escobar, Melissa [1] ; Carneiro Costa, Tassia Brena Barroso [1] ; Martins, Lucas G. [2] ; Costa, Silvia S. [3] ; VanHelvoort Lengert, Andre [4] ; Boldrini, Erica [5] ; Morini da Silva, Sandra Regina [6] ; Fernando Lopes, Luiz [5] ; Onofre Vidal, Daniel [4] ; Krepischi, V, Ana C. ; Maschietto, Mariana [7] ; Tasic, Ljubica [1]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Chem, Dept Organ Chem, Campinas - Brazil
[2] Univ Lima, Fac Ingn Ind, Lima - Peru
[3] V, Univ Sao Paulo, Inst Biosci, Human Genome & Stem Cell Res Ctr CEGH CEL, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[4] Barretos Canc Hosp, Mol Oncol Res Ctr CPOM, Barretos - Brazil
[5] Barretos Childrens Canc Hosp, Barretos - Brazil
[6] Barretos Canc Hosp, Dept Pathol, Barretos - Brazil
[7] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN ONCOLOGY; v. 10, OCT 16 2020.
Citações Web of Science: 0
Resumo

Pediatric osteosarcoma outcomes have improved over the last decades; however, patients who do not achieve a full resection of the tumor, even after aggressive chemotherapy, have the worst prognosis. At a genetic level, osteosarcoma presents many alterations, but there is scarce information on alterations at metabolomic levels. Therefore, an untargeted nuclear magnetic resonance metabonomic approach was used to reveal blood serum alterations, when samples were taken from 21 patients with osteosarcoma aged from 12-20 (18, 86%) to 43 (3, 14%) years before any anticancer therapy were collected. The results showed that metabolites differed greatly between osteosarcoma and healthy control serum samples, especially in lipids, aromatic amino acids (phenylalanine and tyrosine), and histidine concentrations. Besides, most of the loading plots point to protons of the fatty acyls (-CH3 and -CH2-) from very-low- and low-density lipoproteins and cholesterol, as crucial metabolites for discrimination of the patients with osteosarcoma from the healthy samples. The relevance of blood lipids in osteosarcoma was highlighted when analyzed together with the somatic mutations disclosed in tumor samples from the same cohort of patients, where six genes linked to the cholesterol metabolism were found being altered too. The high consistency of the discrimination between osteosarcoma and healthy control blood serum suggests that nuclear magnetic resonance could be successfully applied for osteosarcoma diagnostic and prognostic purposes, which could ameliorate the clinical efficacy of therapy. (AU)

Processo FAPESP: 18/21047-9 - Câncer na infância - predisposição genética e mecanismos de origem
Beneficiário:Ana Cristina Victorino Krepischi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/06510-4 - Aplicação de Ressonância Magnética Nuclear (RMN) em metabolômica e metabonômica de doenças humanas
Beneficiário:Ljubica Tasic
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/06281-7 - Investigação da regulação epigenética em tumores sólidos pediátricos
Beneficiário:Mariana Camargo Maschietto
Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores
Processo FAPESP: 14/10250-7 - Investigação da regulação epigenética em tumores sólidos pediátricos
Beneficiário:Mariana Camargo Maschietto
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores