Brevipalpus Species Vectoring Citrus Leprosis Virus (Cilevirus and Dichorhavirus)

Autor(es)

Ferreira, Leticia M. ^[1] ; Nunes, Maria A. ^[1] ; Sinico, Thais E. ^{[2, 1]} ; Soares, Alex J. ^[1] ; Novelli, Valdenice M. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr Avancado Pesquisa Citros Sylvia Moreira, BR-13490970 Cordeiropolis, SP - Brazil

^[2] Inst Biol, BR-04014900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Economic Entomology; v. 113, n. 4, p. 1628-1634, AUG 2020.
• Citações Web of Science: 0

Resumo

Citrus leprosis (CL) is one of the most devastating viral diseases of orchards, and industries correspondingly invest highly in the management and control of the virus vector. In Brazil, the disease is caused most predominantly by the citrus leprosis virus C (CiLV-C, Kitaviridae: Cilevirus), and also by citrus leprosis virus N (CiLV-N, Rhabdoviridae: Dichorhavirus). Both viruses are transmitted by false spider mites and at least three different species, Brevipalpus yothersi Baker, B. papayensis Baker, and B. phoenicis (Geijskes) sensu stricto, have been reported in citrus orchards. The main goal of this study was to evaluate the capacity of three Brevipalpus species to transmit citrus leprosis virus (cytoplasmic and nuclear types). The capacity of false spider mites to acquire the virus was accomplished using RT-PCR and the ability to inoculation the virus to host plants (common bean and sweet orange) was assessed via viral transmission assays. Common beans infested with B. yothersi and B. papayensis showed symptoms of CiLV-C in 87.5 and 17% of the plants assessed, respectively. In sweet orange, B. yothersi was exclusively able to inoculate CiLV-C, and around 83% of samples were symptomatic. Host plants infected with CiLV-N showed symptoms only when infested with B. phoenicis sensu stricto (s.s.). All the Brevipalpus species (Acari: Tenuipalpidae) were able to acquire both viruses (CiLV-C and CiLV-N), but not infect plants. These results suggest the existence of virus-vector specificity in the leprosis pathosystem, and this information will be critical for enhancing our further understanding of epidemiological features and disease management. (AU)

• Processo FAPESP: 14/08458-9 - Vírus de plantas transmitidos por Brevipalpus (Acari: Tenuipalpidae) - VTB: levantamento, identificação, caracterização molecular, filogenia; relações vírus/vetor/hospedeira; biologia, taxonomia e manejo do vetor
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23464-8 - Avaliação da interação vírus da leprose dos citros (CiLV-C) e diferentes espécies de Brevipalpus spp
• Beneficiário: Leticia Maria Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica