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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Assessment of the Bioactive Potential of Cheese Whey Protein Hydrolysates Using Immobilized Alcalase

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Autor(es):
da Cruz, Clariana Zanutto Paulino [1] ; de Mendonca, Ricardo Jose [2] ; Guimaraes, Luis Henrique Souza [3] ; dos Santos Ramos, Matheus Aparecido [4] ; Garrido, Saulo Santesso [1] ; de Paula, Ariela Veloso [4] ; Monti, Rubens [4] ; Massolini, Gabriella [5]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Chem Inst, BR-14800060 Araraquara, SP - Brazil
[2] Fed Univ Triangulo Mineiro UFTM, Biochem Sect, BR-38015050 Uberaba, MG - Brazil
[3] Sao Paulo Univ USP, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil
[5] Univ Pavia, Dipartimento Sci Farm, I-27100 Pavia, PV - Italy
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Food and Bioprocess Technology; v. 13, n. 12 NOV 2020.
Citações Web of Science: 0
Resumo

The hydrolysis of bovine cheese whey (BCW) proteins was performed by the Alcalase immobilized on the support glyoxyl-corn-cob-powder (AGCCP). The whey protein hydrolysates (WPH) obtained with AGCPP were fractionated by RP-HPLC according to the hydrophilicity of the peptides (F1, hydrophilic; F2, intermediate hydrophilicity; and F3, hydrophobic). The fractions were analyzed by MALDI-TOF, and molecular weight peptides (< 1500 m/z) were identified. Total WPH obtained by AGCCP presented high capacity reduction of the ABTS radical (57.82%) and high chelating activity of iron II (76.2%). The obtained peptides showed high antimicrobial activity (87.75-100%) against the species Escherichia coli (ATCC 43895) and Listeria monocytogenes (ATCC 7644). Only F2 was effective against the Candida albicans strain ATCC 18804 tested (MIC = 10 mg/mL). The results of this study show that the hydrolysis of BCW proteins using the AGCCP derivative represents a very interesting and viable way for obtention of bioactive peptides from cheese whey. (AU)

Processo FAPESP: 12/07680-4 - Hidrolisados parciais de soroproteínas lácteas bovina e bubalina obtidos com proteases imobilizadas: ensaios de transporte e absorção de peptídeos através das técnicas da dialisabilidade e cultura de células Caco-2
Beneficiário:Juliana Cristina Bassan
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/12563-2 - Utilização de resíduos agroindustriais para imobilização e estabilização de enzimas de interesse biotecnológico
Beneficiário:Rubens Monti
Linha de fomento: Auxílio à Pesquisa - Regular