Assessment of the Bioactive Potential of Cheese Whey Protein Hydrolysates Using Immobilized Alcalase

Autor(es)

da Cruz, Clariana Zanutto Paulino ^[1] ; de Mendonca, Ricardo Jose ^[2] ; Guimaraes, Luis Henrique Souza ^[3] ; dos Santos Ramos, Matheus Aparecido ^[4] ; Garrido, Saulo Santesso ^[1] ; de Paula, Ariela Veloso ^[4] ; Monti, Rubens ^[4] ; Massolini, Gabriella ^[5]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Chem Inst, BR-14800060 Araraquara, SP - Brazil

^[2] Fed Univ Triangulo Mineiro UFTM, Biochem Sect, BR-38015050 Uberaba, MG - Brazil

^[3] Sao Paulo Univ USP, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Sao Paulo, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[5] Univ Pavia, Dipartimento Sci Farm, I-27100 Pavia, PV - Italy

• Tipo de documento: Artigo Científico
• Fonte: Food and Bioprocess Technology; v. 13, n. 12 NOV 2020.
• Citações Web of Science: 0

Resumo

The hydrolysis of bovine cheese whey (BCW) proteins was performed by the Alcalase immobilized on the support glyoxyl-corn-cob-powder (AGCCP). The whey protein hydrolysates (WPH) obtained with AGCPP were fractionated by RP-HPLC according to the hydrophilicity of the peptides (F1, hydrophilic; F2, intermediate hydrophilicity; and F3, hydrophobic). The fractions were analyzed by MALDI-TOF, and molecular weight peptides (< 1500 m/z) were identified. Total WPH obtained by AGCCP presented high capacity reduction of the ABTS radical (57.82%) and high chelating activity of iron II (76.2%). The obtained peptides showed high antimicrobial activity (87.75-100%) against the species Escherichia coli (ATCC 43895) and Listeria monocytogenes (ATCC 7644). Only F2 was effective against the Candida albicans strain ATCC 18804 tested (MIC = 10 mg/mL). The results of this study show that the hydrolysis of BCW proteins using the AGCCP derivative represents a very interesting and viable way for obtention of bioactive peptides from cheese whey. (AU)

• Processo FAPESP: 12/07680-4 - Hidrolisados parciais de soroproteínas lácteas bovina e bubalina obtidos com proteases imobilizadas: ensaios de transporte e absorção de peptídeos através das técnicas da dialisabilidade e cultura de células Caco-2
• Beneficiário: Juliana Cristina Bassan
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/12563-2 - Utilização de resíduos agroindustriais para imobilização e estabilização de enzimas de interesse biotecnológico
• Beneficiário: Rubens Monti
• Linha de fomento: Auxílio à Pesquisa - Regular