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Sugarcane mosaic virus mediated changes in cytosine methylation pattern and differentially transcribed fragments in resistance-contrasting sugarcane genotypes

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da Silva, Marcel Fernando [1, 2] ; Goncalves, Marcos Cesar [3] ; Brito, Michael dos Santos [4] ; Medeiros, Cibele Nataliane [1] ; Harakava, Ricardo [3] ; de Andrade Landell, Marcos Guimaraes [1] ; Pinto, Luciana Rossini [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Inst Agron, Ctr Cana, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet FCAV, Biol Aplicada Agr, Jaboticabal, SP - Brazil
[3] Inst Biol, Crop Protect Res Ctr, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Ciencia & Tecnol, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 15, n. 11 NOV 9 2020.
Citações Web of Science: 0

Sugarcane mosaic virus (SCMV) is the causal agent of sugarcane mosaic disease (SMD) in Brazil; it is mainly controlled by using resistant cultivars. Studies on the changes in sugarcane transcriptome provided the first insights about the molecular basis underlying the genetic resistance to SMD; nonetheless, epigenetic modifications such as cytosine methylation is also informative, considering its roles in gene expression regulation. In our previous study, differentially transcribed fragments (DTFs) were obtained using cDNA-amplified fragment length polymorphism by comparing mock- and SCMV-inoculated plants from two sugarcane cultivars with contrasting responses to SMD. In this study, the identification of unexplored DTFs was continued while the same leaf samples were used to evaluate SCMV-mediated changes in the cytosine methylation pattern by using methylation-sensitive amplification polymorphism. This analysis revealed minor changes in cytosine methylation in response to SCMV infection, but distinct changes between the cultivars with contrasting responses to SMD, with higher hypomethylation events 24 and 72 h post-inoculation in the resistant cultivar. The differentially methylated fragments (DMFs) aligned with transcripts, putative promoters, and genomic regions, with a preponderant distribution within CpG islands. The transcripts found were associated with plant immunity and other stress responses, epigenetic changes, and transposable elements. The DTFs aligned with transcripts assigned to stress responses, epigenetic changes, photosynthesis, lipid transport, and oxidoreductases, in which the transcriptional start site is located in proximity with CpG islands and tandem repeats. Real-time quantitative polymerase chain reaction results revealed significant upregulation in the resistant cultivar of aspartyl protease and VQ protein, respectively, selected from DMF and DTF alignments, suggesting their roles in genetic resistance to SMD and supporting the influence of cytosine methylation in gene expression. Thus, we identified new candidate genes for further validation and showed that the changes in cytosine methylation may regulate important mechanisms underlying the genetic resistance to SMD. (AU)

Processo FAPESP: 12/15060-6 - Identificação de genes candidatos para resistência ao mosaico da cana-de-açúcar a partir da técnica de cDNA-AFLP.
Beneficiário:Cibele Nataliane Facioli Medeiros
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 08/56146-5 - (bione/pronex FAPESP) sustainable bioenergy sugarcane breeding and cultivar development
Beneficiário:Marcos Guimarães de Andrade Landell
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático