Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study

Autor(es):Mostrar menos -

Cunha, M. A. ^{[1, 2]} ; Celeste, B. J. ^[3] ; Kesper, N. ^[4] ; Fugimori, M. ^[3] ; Lago, M. M. ^[5] ; Ibanes, A. S. ^[5] ; Ouki, L. M. ^[5] ; Simoes Neto, E. A. ^[6] ; Fonseca, F. F. ^[7] ; Silva, M. A. L. ^[8] ; Barbosa Junior, W. L. ^[8] ; Lindoso, J. A. L. ^{[2, 4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Norte, Dept Infect Dis, Rua Conego Monte 110, BR-59037170 Natal, RN - Brazil

^[2] Univ Sao Paulo, Dept Infect & Parasit Dis, Med Sch, Sao Paulo, SP - Brazil

^[3] Inst Trop Med Sao Paulo, Lab Seroepidemiol & Immunobiol, Sao Paulo, SP - Brazil

^[4] Inst Trop Med Sao Paulo, Lab Protozool, Sao Paulo, SP - Brazil

^[5] Emilio Ribas Inst Infectol, Outpatient Sect, Sao Paulo, SP - Brazil

^[6] Univ Fed Maranhao, Dept Med Course, Pinheiro, Maranhao - Brazil

^[7] Minist Hlth Brazil, Dept Dis Chron Condit & Sexually Transmitted Infe, Brasilia, DF - Brazil

^[8] Aggeu Magalhaes Res Ctr, Parasitol, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC INFECTIOUS DISEASES; v. 20, n. 1 DEC 25 2020.
• Citações Web of Science: 0

Resumo

BackgroundThere is little information about the frequency of Leishmania infection in asymptomatic people living with HIV (PLWH) and about the performance of laboratory diagnostic methods in coinfected patients in Latin America. The main objective of this study is to evaluate the frequency of Leishmania spp. infection in HIV-infected patients living in an urban area in Brazil.MethodsTo detect Leishmania infection, diagnostic tests were performed to detect anti-Leishmania antibodies (ELISA using Leptomonas seymouri antigens; ELISA using rK39 antigens; ELISA using rK28 antigens; indirect fluorescent-antibody test (IFAT); direct agglutination test (DAT)) and Leishmania DNA (polymerase chain reaction (PCR) with the target genes kDNA and ITS-1).ResultsThe frequency of at least one positive test was 15%. For ELISA using Leptomonas antigens and IFAT, there was an association between CD4+ T lymphocyte counts and test positivity, with a higher positivity of these tests in more immunosuppressed patients (CD4+ T cell count <200/mm(3)).ConclusionsAccording to our data, there was a high prevalence of Leishmania spp. infections in this population living with HIV. Although there is the possibility of cross-reaction, some tests that are considered highly specific for the diagnosis of Leishmania infection were positive. There was also an association between the positivity of some tests studied and lower values of CD4+ T lymphocytes. (AU)

• Processo FAPESP: 16/03165-9 - Coinfecção Leishmania/HIV-AIDS: Avaliação da prevalência da coinfecção e avaliação do perfil genotípico de Leishmania (Leishmania) infantum chagasi.
• Beneficiário: José Angelo Lauletta Lindoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/06535-9 - Prevalência da infecção por Leishmania infantum em pacientes com HIV/AIDS provenientes de áreas de transmissão de Leishmaniose visceral
• Beneficiário: Mirella Alves da Cunha
• Modalidade de apoio: Bolsas no Brasil - Mestrado