Moderate, but Not Excessive, Training Attenuates Autophagy Machinery in Metabolic Tissues

Autor(es):Mostrar menos -

da Rocha, Alisson L. ^[1] ; Pinto, Ana P. ^[1] ; Morais, Gustavo P. ^[1] ; Marafon, Bruno B. ^[2] ; Rovina, Rafael L. ^[2] ; Veras, Allice S. C. ^[3] ; Teixeira, Giovana R. ^{[3, 4]} ; Pauli, Jose R. ^[5] ; de Moura, Leandro P. ^[5] ; Cintra, Dennys E. ^[5] ; Ropelle, Eduardo R. ^[5] ; Rivas, Donato A. ^[6] ; da Silva, Adelino S. R. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, BR-14040900 Ribeirao Preto, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Postgrad Program Movement Sci, BR-19060900 Presidente Prudente, SP - Brazil

^[4] State Univ Sao Paulo UNESP, Dept Phys Educ, BR-19060900 Presidente Prudente, SP - Brazil

^[5] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, BR-13484350 Limeira, SP - Brazil

^[6] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 - USA

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 22 NOV 2020.
• Citações Web of Science: 0

Resumo

The protective effects of chronic moderate exercise-mediated autophagy include the prevention and treatment of several diseases and the extension of lifespan. In addition, physical exercise may impair cellular structures, requiring the action of the autophagy mechanism for clearance and renovation of damaged cellular components. For the first time, we investigated the adaptations on basal autophagy flux in vivo in mice's liver, heart, and skeletal muscle tissues submitted to four different chronic exercise models: endurance, resistance, concurrent, and overtraining. Measuring the autophagy flux in vivo is crucial to access the functionality of the autophagy pathway since changes in this pathway can occur in more than five steps. Moreover, the responses of metabolic, performance, and functional parameters, as well as genes and proteins related to the autophagy pathway, were addressed. In summary, the regular exercise models exhibited normal/enhanced adaptations with reduced autophagy-related proteins in all tissues. On the other hand, the overtrained group presented higher expression of Sqstm1 and Bnip3 with negative morphological and physical performance adaptations for the liver and heart, respectively. The groups showed different adaptions in autophagy flux in skeletal muscle, suggesting the activation or inhibition of basal autophagy may not always be related to improvement or impairment of performance. (AU)

• Processo FAPESP: 17/17646-1 - MicroRNAs circulantes como novos preditores de resposta anabólica do músculo esquelético em seres humanos idosos
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 17/19869-8 - Mecanismos moleculares relacionados ao aumento do conteúdo hepático de gordura em resposta ao excesso de exercício físico
• Beneficiário: Ana Paula Pinto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/00855-2 - Sarcopenia, nutrição e atividade física
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 17/09038-1 - Efeitos do exercício físico regular e do overtraining no comportamento da via autofágica em diferentes tecidos de camundongos.
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/12765-2 - Papel emergente da rev-erb-alfa nas adaptações moleculares a diferentes modelos de exercício físico
• Beneficiário: Alisson Luiz da Rocha
• Modalidade de apoio: Bolsas no Brasil - Doutorado