Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods

Autor(es)

Reimao, Juliana Quero ^[1] ; Coser, Elizabeth Magiolo ^[2] ; Lee, Monica Ran ^[1] ; Coelho, Adriano Cappellazzo ^[2]

Afiliação do(s) autor(es)

^[1] Fac Med Jundiai, Dept Morfol & Patol Basicas, BR-13202550 Jundiai - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Anim, BR-13083862 Campinas - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: MICROORGANISMS; v. 8, n. 11 NOV 2020.
• Citações Web of Science: 0

Resumo

Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed. (AU)

• Processo FAPESP: 16/21171-6 - Paromomicina no tratamento da Leishmaniose Tegumentar: investigação in vitro, in vivo e na identificação de marcadores moleculares associados à suscetibilidade e resistência
• Beneficiário: Adriano Cappellazzo Coelho
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 18/18954-4 - Reposicionamento de fármacos: avaliação de atividade, seletividade e mecanismo de ação de novos candidatos contra Toxoplasma gondii
• Beneficiário: Juliana Quero Reimão Dalla Zanna
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores