Does leukotriene F4 play a major role in the infection mechanism of Candida sp.?

Autor(es)

Odir Rodrigues Melo, Carlos Fernando ^[1] ; Bachur, Luis Felipe ^[2] ; Delafiori, Jeany ^[1] ; Dabaja, Mohamed Ziad ^[1] ; de Oliveira, Diogo Noin ^[1] ; Guerreiro, Tatiane Melina ^[1] ; Tararam, Cibele Aparecida ^[3] ; Busso-Lopes, Ariane Fidelis ^[4] ; Moretti, Maria Luiza ^[5] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Pharmaceut Sci, Innovare Biomarkers Lab, BR-13083877 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Hosp & Clin, Div Hosp Epidemiol, BR-13083888 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Mol Epidemiol & Infect Dis Lab, BR-13083888 Campinas, SP - Brazil

^[4] Brazilian Biosci Natl Lab, BR-13083970 Sao Paulo - Brazil

^[5] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, BR-13083888 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 149, DEC 2020.
• Citações Web of Science: 0

Resumo

Candidiasis is the most common fungal infection affecting hospitalized patients, especially immunocompromised and critical patients. Limitations regarding the assertive diagnosis of both Candidemia and Candidiasis not only impairs the introduction of effective treatments but also lays a heavy financial burden over the health system. Furthermore, it is still challenging to ascertain whether diagnostic methods are accurate and whether treatment is effective for patients with Candidemia. These constraints come from the uncertainty of the pathophysiological mechanism by which the pathogen establishes the opportunistic infection. Additionally, it is the reason why some patients present positive blood culture results, and others do not, and why it is very difficult during clinical routines to prove Candidemia or invasive candidiasis. Taking into account the current situation, this contribution proposes two markers that may help to understand the mechanisms of infection by the pathogen: Leukotriene F4 and 5,6-dihydroxy-eicosatetraenoic. These two lipids putatively modulate the host's immune response, and the initial data presented in this contribution suggest that these lipids allow the opportunistic infection to be installed. The study was carried out using an omics-based platform using direct-infusion high-resolution mass spectrometry and allied with bioinformatics tools to provide accurate and reliable results for biomarker candidates screening. (AU)

• Processo FAPESP: 16/17066-2 - Metabolômica e lipidômica do Zika vírus, do mosquito ao paciente
• Beneficiário: Carlos Fernando Odir Rodrigues Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/03321-6 - Uma visão geral da metabolômica e lipidômica do plasma de pacientes com Doença de Alzheimer para rastreamento de biomarcadores ligados à demência
• Beneficiário: Carlos Fernando Odir Rodrigues Melo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/06809-1 - Determinação de marcadores químicos em diferentes produtos bovinos por Espectrometria de Massas
• Beneficiário: Rodrigo Ramos Catharino
• Modalidade de apoio: Auxílio à Pesquisa - Regular