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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of berberine associated with photodynamic therapy in cell lines

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Autor(es):
Oliveira, P. M. [1] ; Lopes, T. Z. [2] ; Tedesco, A. C. [3] ; Rahal, P. [2] ; Calmon, M. F. [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Fed Pelotas UFPel, Fed Univ Pelotas, Ctr Desenvolvimento Tecnol CDTec, Technol Dev Ctr, Pelotas, RS - Brazil
[2] Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci Humanities & Exact Sci IBILCE, S ao Jose Do Rio Preto - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Ctr Nanotechnol & Tissue Engn, Photobiol Photomed, Ribeirao Preto - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 32, DEC 2020.
Citações Web of Science: 0
Resumo

Cervical cancer is a serious worldwide health problem. In view of the potentially harmful effects of current conventional therapies, photodynamic therapy may be an option as it is a minimally invasive therapy and can promote selective cytotoxic activity for neoplastic cells in the target tissue., Berberine (BBR) as an isolated molecule is a natural compound that has antineoplastic properties and potential action as a photosensitizer agent. The purpose of this study was to evaluate the use of berberine as a photosensitizer in photodynamic therapy (PDT) protocols and observe the effects produced by this association in cervical carcinoma cells and in immortalized keratinocytes. Incubation with 2.5 mu M berberine promoted less than 10 % of cellular death in both cell lines studied. In addition, by fluorescence microscopy, we demonstrated that berberine was internalized by the cells, and after a period of 48 h, it was still present in the intracellular environment preferentially localized in the cytoplasm. After photodynamic therapy using berberine as a photosensitizer and visible light activation at 447 (+/- 10) nm, we observed a phototoxic effect, which resulted in 19.84 % cell viability for Caski cells and 47.22 % cell viability for HaCaT. Treatment with berberine associated with photodynamic therapy promoted an increase in the production of reactive species of oxygen (ROS) and caspase-3 activity, indicating a preferential cell death mechanism by caspase-dependent apoptosis. Therefore, we demonstrated that berberine is an efficient photosensitizer and that its association with photodynamic therapy may be a potential anticancer treatment strategy for cervical cancer. (AU)

Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/16660-5 - Estudo de nanoemulsões associadas a terapia fotodinâmica no tratamento do câncer de mama
Beneficiário:Marilia de Freitas Calmon
Modalidade de apoio: Auxílio à Pesquisa - Regular