Sesquiterpene Polygodial from Drimys brasiliensis (Winteraceae) Down-Regulates Implant-Induced Inflammation and Fibrogenesis in Mice

Autor(es)

Ferreira, Bruno Antonio ^[1] ; Norton Filho, Anderson Ferraz ^[2] ; Deconte, Simone Ramos ^[2] ; Tomiosso, Tatiana Carla ^[3] ; Thevenard, Fernanda ^[4] ; Andrade, Silvia Passos ^[5] ; Ghilardi Lago, Joao Henrique ^[4] ; Araujo, Fernanda de Assis ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia UFU, Inst Biotecnol, Programa Posgrad Genet & Bioquim, BR-38408100 Uberlandia, MG - Brazil

^[2] Univ Fed Uberlandia UFU, Inst Ciencias Biomed, Dept Ciencias Fisiol, BR-38408100 Uberlandia, MG - Brazil

^[3] Univ Fed Uberlandia UFU, Inst Ciencias Biomed, Dept Biol Celular Histol & Embriol, BR-38408100 Uberlandia, MG - Brazil

^[4] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

^[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Fisiol & Biofis, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 83, n. 12, p. 3698-3705, DEC 24 2020.
• Citações Web of Science: 0

Resumo

Drimys brasiliensis (Winteraceae) has been investigated in traditional medicine for its anti-inflammatory properties to treat gastric ulcers and allergic and respiratory system diseases as well as for cancer treatment. In this work, we investigate the ability of the sesquiterpene polygodial, isolated from D. brasiliensis stem barks, to modulate the chronic inflammatory response induced by polyester-polyurethane sponge implants in C57BL/6J mice. Daily treatment with polygodial inhibited the macrophage content in the implants as determined by the activity of the N-acetyl-beta-D-glucosaminidase enzyme as well as decreased the levels of CXCL1/KC and CCL2/JE/MCP-1 pro-inflammatory chemokines and the presence of mast cells along the formed fibrovascular tissue. Similarly, the deposition of a new extracellular matrix (total collagen and type I and III collagen fibers) as well as the production of the TGF-beta 1 cytokine were attenuated in implants treated with polygodial, showing for the first time its antifibrogenic capacity. The hemoglobin content, the number of newly formed vessels, and the levels of VEGF cytokine, which were used as parameters for the assessment of the neovascularization of the implants, did not change after treatment with polygodial. The anti-inflammatory and antifibrogenic effects of polygodial over the components of the granulation tissue induced by the sponge implant indicate a therapeutic potential for the treatment of inflammatory diseases associated with the development of fibrovascular tissue. (AU)

• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular