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Effects of melatonin on insulin signaling and inflammatory pathways of rats with apical periodontitis

Texto completo
Autor(es):
Tavares, B. S. [1] ; Tsosura, T. V. S. [1] ; Mattera, M. S. L. C. [1] ; Santelli, J. O. [1] ; Belardi, B. E. [1] ; Chiba, F. Y. [2] ; Cintra, L. T. A. [2] ; Silva, C. C. [2] ; Matsushita, D. H. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, PPGMCF, Programa Posgrad Multicentr Ciencias Fisiol, Dept Basic Sci, SBFis, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Prevent & Restorat Dent, Aracatuba - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: International Endodontic Journal; JAN 2021.
Citações Web of Science: 0
Resumo

Aim To verify the effects of melatonin supplementation on insulin sensitivity, plasma concentrations of inflammatory cytokines, insulin signalling and inflammatory pathways in the soleus (SM) and extensor digitorum longus (EDL) muscles of rats with apical periodontitis (AP). Methodology Seventy-two Wistar rats were distributed into 4 groups: (a) control (C), (b) control supplemented with melatonin (M), (c) AP (AP), and (d) AP supplemented with melatonin (AP + M). AP was induced by pulp exposure of the maxillary and mandibular right first and second molars to the oral environment. After AP induction, oral supplementation with 5 mg kg(-1) melatonin (diluted in drinking water) for 60 days was initiated. At the end of the treatment, the following were analysed: (1) plasma concentrations of insulin and inflammatory cytokines (TNF-alpha, IL-6, IL-1 beta and IL-10) using ELISA kits; (2) glycaemia using enzymatic assay; (3) insulin resistance using homoeostasis model assessment of insulin resistance (HOMA-IR) index; and (4) phosphorylation status of pp185 tyrosine, Akt serine, IKK alpha/beta, and JNK in SM and EDL using Western blot. Analysis of variance of two or three factors was performed, followed by the Bonferroni test. P values AP promoted insulin resistance, significantly increased (P < 0.05) plasma concentrations of pro-inflammatory cytokines (TNF-alpha, IL-6, and IL-1 beta), significantly decreased (P < 0.05) the concentration of anti-inflammatory cytokine IL-10, impaired insulin signalling in SM, and increased IKK alpha/beta phosphorylation status in SM and EDL. Melatonin supplementation in rats with AP improved insulin sensitivity, significantly decreased (P < 0.05) TNF-alpha and IL-1 beta, significantly increased (P < 0.05) IL-10 plasma concentrations, and changed the insulin signalling in soleus muscle and IKK alpha/beta phosphorylation status in SM and EDL muscles. Conclusions Melatonin is a potent adjuvant treatment for improving apical periodontitis-associated changes in insulin sensitivity, insulin signalling and inflammatory pathways. In addition, the negative impact of AP on general health was also demonstrated. (AU)

Processo FAPESP: 18/23346-3 - Efeitos da melatonina na via insulínica e inflamatória de ratos com periodontite apical
Beneficiário:Bruna Soares Tavares
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 19/12995-3 - Efeitos da melatonina sobre a sensibilidade insulínica e a etapa inicial do sinal insulínico em ratos com periodontite apical
Beneficiário:Julia Ogata Santelli
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 19/18589-7 - Avaliação da sensibilidade à insulina, concentrações plasmáticas de citocinas inflamatórias, perfil lipídico e vias insulínica e inflamatória em ratos com periodontite apical tratados com melatonina
Beneficiário:Doris Hissako Sumida
Linha de fomento: Auxílio à Pesquisa - Regular