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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The nonpharmacological sequence method provides a reliable evaluation of baroreflex sensitivity in fish

Texto completo
Autor(es):
Armelin, Vinicius A. [1, 2, 3] ; da Silva Braga, Victor H. [2, 3] ; Teixeira, Mariana T. [2, 3] ; Guagnoni, Igor N. [2, 3] ; Wang, Tobias [3, 4] ; Florindo, Luiz H. [2, 3, 5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Physiol, Sao Paulo, SP - Brazil
[2] Sao Paulo State Univ, Dept Zool & Bot, UNESP, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Natl Inst Sci & Technol Comparat Physiol INCT FAP, Rio Claro, SP - Brazil
[4] Aarhus Univ AU, Dept Biosci, Sect Zoophysiol, Aarhus - Denmark
[5] Sao Paulo State Univ, Aquaculture Ctr CAUNESP, UNESP, Jaboticabal, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY; v. 335, n. 3 JAN 2021.
Citações Web of Science: 0
Resumo

The most commonly used technique to study the barostatic regulation of blood pressure in ectothermic vertebrates consists of determining the heart rate response to pharmacological manipulations of blood pressure, the so-called ``Oxford method.{''} Although well established, the Oxford method has some important limitations, such as induction of hypervolemia in small animals and undesired effects of vasoactive drugs on central and peripheral baroreflex components. As an alternative, the sequence method, which consists in the computerized evaluation of naturally-occurring baroreflex adjustments of heart rate without the need for pharmacological administrations, was developed to study baroreflexes. In the present study, we compare this sequence method with the Oxford technique in two teleost species with different life styles, and we assess the optimal software configuration for the employment of the sequence method in fish. Calculation of baroreflex gain through the sequence method was adequate and reliable when the software was configured to search for baroreflex sequences with a minimum length of three cardiac cycles with a delay of one cardiac cycle between fluctuations in mean ventral aortic blood pressure and reflex changes in pulse interval. When properly configured, the sequence and the Oxford methods yielded similar determinations of the baroreflex gain in fish. (AU)

Processo FAPESP: 16/17572-5 - A Modulação Cardíaca Parassimpática no Tambaqui (Colossoma macropomum): Influências dos Ramos Vagais Direito e Esquerdo
Beneficiário:Victor Hugo da Silva Braga
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/57712-4 - Instituto Nacional de Pesquisas em Fisiologia Comparada
Beneficiário:Augusto Shinya Abe
Linha de fomento: Auxílio à Pesquisa - Temático