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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products

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Autor(es):
Chiba, Camila Hiromi [1] ; Knirsch, Marcos Camargo [1] ; Azzoni, Adriano Rodrigues [2] ; Moreira, Antonio R. [3] ; Stephano, Marco Antonio [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Tecnol Bioquim Farmaceut, Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Sao Paulo - Brazil
[3] Univ Maryland Baltimore Cty, Dept Chem Biochem & Environm Engn, Baltimore, MD 21228 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo de Revisão
Fonte: BIOTECHNIQUES; v. 70, n. 2 JAN 2021.
Citações Web of Science: 0
Resumo

Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with `difficult-to-express' proteins. Cell-free protein synthesis (CFPS) can overcome the main bottlenecks of cell-based expression. This review aims to present recent advances in the production process of biologic products by CFPS. First, key aspects of CFPS systems are summarized. A description of several biologic products that have been successfully produced using the CFPS system is provided. Finally, the CFPS system's ability to scale up and scale down, its main limitations and its application for biologics production are discussed. (AU)

Processo FAPESP: 18/15794-6 - PRODUÇÃO DA PROTEÍNA RECOMBINANTE INTERFERON beta 1B NA PLATAFORMA DE SÍNTESE PROTEICA LIVRE DE CÉLULA
Beneficiário:Marco Antonio Stephano
Linha de fomento: Auxílio à Pesquisa - Regular