Polymorphisms and haplotypes in the Interleukin 17 Alfa gene: potential effect of smoking habits in the association with periodontitis and type 2 diabetes mellitus

Autor(es)

Rimachi Hidalgo, Marco A. ^{[1, 2]} ; Cirelli, Thamiris ^{[1, 2]} ; da Silva, Barbara Roque ^{[1, 2]} ; Nicchio, Ingra Gagno ^{[1, 2]} ; Nepomuceno, Rafael ^{[1, 2]} ; Orrico, Silvana R. P. ^{[1, 3]} ; Cirelli, Joni A. ^[1] ; Theodoro, Leticia Helena ^[1] ; Barros, Silvana P. ^[4] ; Scarel-Caminaga, Raquel M. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent Araraquara, Dept Morphol & Pediat Clin, Araraquara, SP - Brazil

^[3] Union Coll Great Lakes UNILAGO, Adv Res Ctr Med, BR-15030070 Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ North Carolina Chapel Hill UNC, Sch Dent, Dept Periodontol, Chapel Hill, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 48, n. 2 FEB 2021.
• Citações Web of Science: 0

Resumo

Few studies evaluate interrelationships between periodontitis (P) and Type 2 Diabetes Mellitus (T2DM). The aim of this study is to investigate the genetic susceptibility to periodontitis alone, or concomitant with T2DM (as comorbidities), analyzing single nucleotide polymorphisms (SNPs) in the Interleukin 17 alpha (IL17A) gene, considering the biochemical profile and smoking habits on the subjects' periodontal status. We investigated 879 individuals divided into: T2DM subjects also affected by severe or moderate periodontitis (T2DM-P, n = 199); non-diabetics with severe or moderate periodontitis (PERIODONTITIS, n = 342); and healthy subjects (HEALTHY, n = 338). Subjects underwent complete periodontal examination, history of smoking habits, glycemic and lipid biochemical evaluation. DNA from buccal cells was utilized to genotype the SNPs rs2275913, rs3819024 and rs10484879. The impact of the subjects' biochemical profile was analyzed in their periodontal status. Each SNP was analyzed independently, and as haplotypes, by multiple logistic regressions, adjusted for covariates, and also stratifying the groups by age, sex and smoking habits. Independently of the periodontitis degree, poorly-controlled T2DM subjects showed worse glycemic and lipid profile. Multiple logistic regressions demonstrated that smokers and former-smokers carrying the GG genotype of rs3819024 seemed to have higher risk for T2DM-Periodontitis (OR = 6.33; 95% CI = 1.26-31.77, p = 0.02), and mainly for T2DM alone (OR = 5.11; 95% CI = 1.37-19.06, p = 0.01), than never smokers. We found the potential effect of smoking habits in the association of IL17A-rs3819024-GG with diseased phenotypes. Because the observed wide confidence intervals, further studies enrolling larger populations, and SNPs' functional evaluations are needed to better understand our findings. (AU)

• Processo FAPESP: 18/26367-1 - Investigação da associação de polimorfismos no gene defensina beta1 (DEFB1)com suscetibilidade à doença periodontal crônica e diabetes mellitus tipo 2
• Beneficiário: Ana Cláudia dos Santos Rios
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/03753-8 - Investigação de potenciais marcadores genéticos da periodontite crônica associada ou não ao Diabetes Mellitus tipo 2 por meio da suscetibilidade genética e do perfil glicêmico e lipídico do paciente
• Beneficiário: Raquel Mantuaneli Scarel Caminaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/08070-6 - Investigação da suscetibilidade genética à periodontite crônica associada ao Diabetes mellitus tipo 2
• Beneficiário: Thamiris Cirelli
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/18313-3 - Investigação da susceptibilidade de variações genéticas a Periodontite Crônica por OpenArray®: Bioestatística, Bioinformática e Análise Funcional
• Beneficiário: Rafael Nepomuceno Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/13295-1 - Investigação da associação de variações genéticas na suscetibilidade à doença periodontal crônica por meio de OpenArray®
• Beneficiário: Rafael Nepomuceno Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado