| Texto completo | |
| Autor(es): |
de Freitas Guaitolini, Carlos Renato
[1]
;
Dias Maziero, Rosiara Rosaria
[1]
;
de Souza Castilho, Anthony Cesar
[2]
;
Pagnano Derussi, Ana Augusta
[3]
;
Volpato, Rodrigo
[4]
;
Ackermann, Camila Louise
[4]
;
Matsubara, Lidia Mitsuko
[4]
;
Lopes, Maria Denise
[4]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Paranaense, Umuarama, PR - Brazil
[2] Oeste Paulista Univ, Presidente Prudente, SP - Brazil
[3] Jose do Rosario Vellano Univ, Alfenas, MG - Brazil
[4] FMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CRYOLETTERS; v. 41, n. 6, p. 330-336, NOV 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
BACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation. (AU) | |
| Processo FAPESP: | 12/03912-8 - Efeitos da congelação lenta e vitrificação em embriões caninos |
| Beneficiário: | Maria Denise Lopes |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/08798-6 - Efeitos da congelação lenta e vitrificação na viabilidade, ultraestrutura e expressão gênica de embriões caninos |
| Beneficiário: | Carlos Renato de Freitas Guaitolini |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |