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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach

Texto completo
Autor(es):
Miotto, Danyelle S. [1, 2] ; Dionizio, Aline [3] ; Jacomini, Andre M. [4] ; Zago, Anderson S. [5, 4] ; Buzalaf, Marilia Afonso Rabelo [3] ; Amaral, Sandra L. [1, 2, 5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Joint Grad Program Physiol Sci, Sao Carlos - Brazil
[2] Sao Paulo State Univ, UFSCar UNESP, Sao Carlos - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[4] Sao Paulo State Univ, Postgrad Program Movement Sci, Bauru, SP - Brazil
[5] Sao Paulo State Univ, Sch Sci, Dept Phys Educ, Bauru, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PHYSIOLOGY; v. 12, FEB 10 2021.
Citações Web of Science: 0
Resumo

Arterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness. (AU)

Processo FAPESP: 17/00509-1 - Papel do treinamento físico no balanço autonômico, rigidez arterial e rarefação em ratos espontaneamente hipertensos tratados com dexametasona: contribuição dos microRNAs
Beneficiário:Sandra Lia do Amaral Cardoso
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/12041-7 - Segurança e eficácia para prevenção da erosão dentária de uma nova cistatina derivada da cana-de-açúcar (CaneCPI-5): estudos in vitro, in situ e in vivo
Beneficiário:Marília Afonso Rabelo Buzalaf
Linha de fomento: Auxílio à Pesquisa - Regular