| Texto completo | |
| Autor(es): Mostrar menos - |
Pinto, Ana P.
[1]
;
da Rocha, Alisson L.
[1]
;
Marafon, Bruno B.
[2]
;
Rovina, Rafael L.
[2]
;
Munoz, Vitor R.
[3]
;
da Silva, Lilian E. C. M.
[4]
;
Pauli, Jose R.
[3]
;
de Moura, Leandro P.
[3]
;
Cintra, Dennys E.
[3]
;
Ropelle, Eduardo R.
[3]
;
da Silva, Adelino S. R.
[1, 2]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ave Bandeirantes 3900, BR-14040907 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ave Bandeirantes 3900, BR-14040907 Ribeirao Preto - Brazil
[3] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Rua Pedro Zacarias 1300, BR-13484350 Limeira - Brazil
[4] Univ Sao Paulo, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Sch Med Ribeirao Preto, Ave Bandeirantes 3900, BR-14040907 Ribeirao Preto - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 5 MAR 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Although physical exercise-induced autophagy activation has been considered a therapeutic target to enhance tissue health and extend lifespan, the effects of different exercise models on autophagy in specific metabolic tissues are not completely understood. This descriptive investigation compared the acute effects of endurance (END), exhaustive (ET), strength (ST), and concurrent (CC) physical exercise protocols on markers of autophagy, genes, and proteins in the gastrocnemius muscle, heart, and liver of mice. The animals were euthanized immediately (0 h) and six hours (6 h) after the acute exercise for the measurement of glycogen levels, mRNA expression of Prkaa1, Ppargc1a, Mtor, Ulk1, Becn1, Atg5, Map1lc3b, Sqstm1, and protein levels of Beclin 1 and ATG5. The markers of autophagy were measured by quantifying the protein levels of LC3II and Sqstm1/p62 in response to three consecutive days of intraperitoneal injections of colchicine. In summary, for gastrocnemius muscle samples, the main alterations in mRNA expressions were observed after 6 h and for the ST group, and the markers of autophagy for the CC group were increased (i.e., LC3II and Sqstm1/p62). In the heart, the Beclin 1 and ATG5 levels were downregulated for the ET group. Regarding the markers of autophagy, the Sqstm1/p62 in the heart tissue was upregulated for the END and ST groups, highlighting the beneficial effects of these exercise models. The liver protein levels of ATG5 were downregulated for the ET group. After the colchicine treatment, the liver protein levels of Sqstm1/p62 were decreased for the END and ET groups compared to the CT, ST, and CC groups. These results could be related to diabetes and obesity development or liver dysfunction improvement, demanding further investigations. (AU) | |
| Processo FAPESP: | 17/09038-1 - Efeitos do exercício físico regular e do overtraining no comportamento da via autofágica em diferentes tecidos de camundongos. |
| Beneficiário: | Adelino Sanchez Ramos da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/19869-8 - Mecanismos moleculares relacionados ao aumento do conteúdo hepático de gordura em resposta ao excesso de exercício físico |
| Beneficiário: | Ana Paula Pinto |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/10875-0 - Efeitos do exercício físico regular e do overtraining no comportamento da via autofágica em diferentes tecidos de camundongos |
| Beneficiário: | Bruno Brieda Marafon |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 17/12765-2 - Papel emergente da rev-erb-alfa nas adaptações moleculares a diferentes modelos de exercício físico |
| Beneficiário: | Alisson Luiz da Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |